Question

limma for gene expression analysis with big and unbalanced sample size

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tati6406 • 0

@tati6406-10823

Last seen 6.8 years ago

Hi Dears

I am not very familiar with limma, I want use limma for finding DEGs of two different phenotype

But there are some ambiguous issues for me in using limma for this work. Sample size of my phenotypes are completely different about 100 for control and 800 for case,now I don't know does limma could be use for this work that have big and unbalanced sample size?

limma microarray statistics • 1.2k views

ADD COMMENT • link updated 7 months ago by XW • 0 • written 8.5 years ago by tati6406 • 0

score 2 · Answer 1 · 2016-06-02

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Aaron Lun ★ 28k

@alun

Last seen 2 hours ago

The city by the bay

There are no problems with using limma for unbalanced data sets.

ADD COMMENT • link 8.5 years ago Aaron Lun ★ 28k

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Could you explain more about the comment "there are no problems with using limma for unbalanced data sets" ? thanks.

ADD REPLY • link 7 months ago XW • 0