Hello,
Here is part of my sample sheet. I am wondering if I need more info here to normalize the batch. I have 3 chips in the batch, all the same group, all medulloblastomas.
[Data] | ||||||
Sample_Name | Sample_Well | Sample_Plate | Sample_Group | Pool_ID | Sentrix_ID | Sentrix_Position |
MDB-01 | MDB | 200340580047 | R01C01 | |||
MDB-02 | MDB | 200340580047 | R02C01 | |||
MDB-10 | MDB | 200340580047 | R03C01 | |||
Any help would be appreciated,
Brenna
batchNorm <- champ.runCombat()
Preparing files for ComBat
Your data is being logit transformed before batch correction
Error in `contrasts<-`(`*tmp*`, value = contr.funs[1 + isOF[nn]]) :
contrasts can be applied only to factors with 2 or more levels
> sessionInfo()
R version 3.3.0 (2016-05-03)
Platform: x86_64-w64-mingw32/x64 (64-bit)
Running under: Windows 7 x64 (build 7601) Service Pack 1
locale:
[1] LC_COLLATE=English_United States.1252
[2] LC_CTYPE=English_United States.1252
[3] LC_MONETARY=English_United States.1252
[4] LC_NUMERIC=C
[5] LC_TIME=English_United States.1252
attached base packages:
[1] stats4 parallel stats graphics grDevices utils datasets
[8] methods base
other attached packages:
[1] IlluminaHumanMethylationEPICmanifest_0.3.0
[2] ChAMP_1.10.0
[3] Illumina450ProbeVariants.db_1.8.0
[4] ChAMPdata_1.10.0
[5] minfi_1.18.2
[6] bumphunter_1.12.0
[7] locfit_1.5-9.1
[8] iterators_1.0.8
[9] foreach_1.4.3
[10] Biostrings_2.40.1
[11] XVector_0.12.0
[12] SummarizedExperiment_1.2.2
[13] GenomicRanges_1.24.0
[14] GenomeInfoDb_1.8.2
[15] IRanges_2.6.0
[16] S4Vectors_0.10.1
[17] lattice_0.20-33
[18] Biobase_2.32.0
[19] BiocGenerics_0.18.0
loaded via a namespace (and not attached):
[1] httr_1.1.0
[2] nor1mix_1.2-1
[3] splines_3.3.0
[4] doRNG_1.6
[5] Rsamtools_1.24.0
[6] impute_1.46.0
[7] RSQLite_1.0.0
[8] limma_3.28.5
[9] quadprog_1.5-5
[10] chron_2.3-47
[11] digest_0.6.9
[12] RColorBrewer_1.1-2
[13] qvalue_2.4.2
[14] colorspace_1.2-6
[15] fastICA_1.2-0
[16] preprocessCore_1.34.0
[17] Matrix_1.2-6
[18] plyr_1.8.3
[19] GEOquery_2.38.4
[20] siggenes_1.46.0
[21] XML_3.98-1.4
[22] biomaRt_2.28.0
[23] genefilter_1.54.2
[24] zlibbioc_1.18.0
[25] xtable_1.8-2
[26] scales_0.4.0
[27] RefFreeEWAS_2.0
[28] IlluminaHumanMethylation450kmanifest_0.4.0
[29] BiocParallel_1.6.2
[30] openssl_0.9.3
[31] annotate_1.50.0
[32] beanplot_1.2
[33] pkgmaker_0.22
[34] mgcv_1.8-12
[35] ggplot2_2.1.0
[36] GenomicFeatures_1.24.2
[37] survival_2.39-4
[38] magrittr_1.5
[39] mclust_5.2
[40] RPMM_1.20
[41] doParallel_1.0.10
[42] nlme_3.1-128
[43] MASS_7.3-45
[44] isva_1.8
[45] tools_3.3.0
[46] registry_0.3
[47] data.table_1.9.6
[48] matrixStats_0.50.2
[49] stringr_1.0.0
[50] munsell_0.4.3
[51] cluster_2.0.4
[52] rngtools_1.2.4
[53] AnnotationDbi_1.34.3
[54] base64_2.0
[55] grid_3.3.0
[56] RCurl_1.95-4.8
[57] marray_1.50.0
[58] bitops_1.0-6
[59] DNAcopy_1.46.0
[60] gtable_0.2.0
[61] codetools_0.2-14
[62] multtest_2.28.0
[63] DBI_0.4-1
[64] reshape_0.8.5
[65] reshape2_1.4.1
[66] R6_2.1.2
[67] illuminaio_0.14.0
[68] GenomicAlignments_1.8.0
[69] rtracklayer_1.32.0
[70] wateRmelon_1.16.0
[71] stringi_1.0-1
[72] sva_3.20.0
[73] Rcpp_0.12.5