Entering edit mode
krababah
•
0
@krababah-10712
Last seen 8.4 years ago
Hi,
I just started using CRISPRseek application, thanks to Dr.Julie Zhu to walk me through issues that got resolved.
I called the offTargetAnalysis function as shown below:
offTargetAnalysis(inputFilePath, findgRNAsWithREcutOnly = FALSE, + REpatternFile = REpatternFile, findPairedgRNAOnly = FALSE, BSgenomeName = Hsapiens,txdb = TxDb.Hsapiens.UCSC.hg19.knownGene, max.mismatch = 3, chromToSearch = "",outputDir = outputDir, overwrite = TRUE)
the input file was :
45 GACCCACGCCTGCTCCCTCATCCACTGTGTGCACTTCATCCTGGA rs362331C
then i got results :
A DNAStringSet instance of length 7 width seq names [1] 23 CCACTGTGTGCACTTCATCCTGG rs362331C_gR38f [2] 23 CCAGGATGAAGTGCACACAGTGG rs362331C_gR28r [3] 23 TGAAGTGCACACAGTGGATGAGG rs362331C_gR22r [4] 23 GAAGTGCACACAGTGGATGAGGG rs362331C_gR21r [5] 23 CACACAGTGGATGAGGGAGCAGG rs362331C_gR15r [6] 23 AGTGGATGAGGGAGCAGGCGTGG rs362331C_gR10r [7] 23 GTGGATGAGGGAGCAGGCGTGGG rs362331C_gR9r
beside other *.xls files.
The problem that i have is that i do not know to interpret those results, How can i build needed knowledge that will enable me to read, comprehend those results.
Thanks