Hi all,
I am using goseq to correct for RNA-seq length bias. Since my dog genome (canFam3) is not supported in goseq database, I tried to make TXDb object and GOmap by myself. However, I am not sure if I am doing it correctly.
If it's correct, it could potentially be a tutorial and benefit other users since the goseq documentation isn't very self-explanatory.
I have posted a question in BioStars but I think I could potentially get some help here.
To prevent repetitive posting, here is the link to my R script: https://www.biostars.org/p/191626/
And here is my `sessionInfo()`:
R version 3.2.4 (2016-03-10)
Platform: x86_64-apple-darwin13.4.0 (64-bit)
Running under: OS X 10.11.3 (El Capitan)
locale:
[1] C
attached base packages:
[1] parallel stats4 stats graphics grDevices utils datasets
[8] methods base
other attached packages:
[1] biomaRt_2.26.1 GenomicFeatures_1.22.13
[3] AnnotationDbi_1.32.3 devtools_1.11.1
[5] goseq_1.22.0 RSQLite_1.0.0
[7] DBI_0.4-1 geneLenDataBase_1.6.0
[9] BiasedUrn_1.07 DESeq2_1.10.1
[11] RcppArmadillo_0.6.700.6.0 Rcpp_0.12.4
[13] SummarizedExperiment_1.0.2 Biobase_2.30.0
[15] GenomicRanges_1.22.4 GenomeInfoDb_1.6.3
[17] IRanges_2.4.8 S4Vectors_0.8.11
[19] BiocGenerics_0.16.1
loaded via a namespace (and not attached):
[1] genefilter_1.52.1 locfit_1.5-9.1 splines_3.2.4
[4] lattice_0.20-33 colorspace_1.2-6 mgcv_1.8-12
[7] rtracklayer_1.30.4 chron_2.3-47 survival_2.39-4
[10] XML_3.98-1.4 withr_1.0.1 foreign_0.8-66
[13] BiocParallel_1.4.3 RColorBrewer_1.1-2 lambda.r_1.1.7
[16] plyr_1.8.3 zlibbioc_1.16.0 Biostrings_2.38.4
[19] munsell_0.4.3 gtable_0.2.0 futile.logger_1.4.1
[22] memoise_1.0.0 latticeExtra_0.6-28 geneplotter_1.48.0
[25] acepack_1.3-3.3 xtable_1.8-2 scales_0.4.0
[28] Hmisc_3.17-4 annotate_1.48.0 XVector_0.10.0
[31] Rsamtools_1.22.0 gridExtra_2.2.1 digest_0.6.9
[34] ggplot2_2.1.0 grid_3.2.4 tools_3.2.4
[37] bitops_1.0-6 RCurl_1.95-4.8 Formula_1.2-1
[40] cluster_2.0.4 futile.options_1.0.0 GO.db_3.2.2
[43] Matrix_1.2-6 data.table_1.9.6 rpart_4.1-10
[46] nlme_3.1-128 GenomicAlignments_1.6.3 nnet_7.3-12