Hi Julie,

When I use CRISPRseek to predict off-target sites of Sp gRNA. I keep getting an error like this:

"[1] "MY_ERROR:   Error in library(GeneRfold): there is no package called ‘GeneRfold’\n"
Done. Please check output files in directory..." 

I could still see the offTarget Analysis EXCEL sheet and collect the results. What does this error mean? Are all the results still meaningful?

The scripts I input is:

"Results1 <- offTargetAnalysis(inputFilePath, findgRNAsWithREcutOnly = FALSE, findPairedgRNAOnly = FALSE, gRNA.size = 20, min.score=0, topN=100000, BSgenomeName = Hsapiens, chromToSearch = "all",  PAM = "NGG", PAM.size=3, PAM.pattern="NG[G|A]$",weights = c(0, 0, 0.014, 0, 0, 0.395, 0.317, 0, 0.389, 0.079, 0.445, 0.508, 0.613, 0.851, 0.732, 0.828, 0.615, 0.804, 0.685, 0.583), txdb = TxDb.Hsapiens.UCSC.hg19.knownGene, orgAnn = org.Hs.egSYMBOL, max.mismatch = 6, outputDir = outputDir, overwrite = TRUE)"

Thank you!

Daniel