Hello, I try to fill my missing identified value (NA) with mean between 7 time course experiment. I have .txt file which contains first row as row name, and the first column as label. I could not fill NA value by both coding in R or using Mfuzzgui. The first 5 row from my text file is something like this.

What I did in R (version 3.2.3) and got the error has shown here below

> exprsFile<-"C:/Users/filename.txt"
> PKA <- read.table(exprsFile, sep="\t")
> eset<-new("ExpressionSet",exprs=as.matrix(PKA));
> eset.f<-fill.NA(eset,mode="mean")
There were 50 or more warnings (use warnings() to see the first 50)
> warnings()
Warning messages:
1: In mean.default(data[i, ], na.rm = TRUE) :
  argument is not numeric or logical: returning NA

By command PKA, I got

>PKA
        V1   V2       V3        V4        V5        V6        V7        V8
1    LABEL 0min    15min     30min     60min    180min    360min    720min
2  1787    0    41502     72899    138534      <NA>    442429      <NA>
3  1763    0     <NA>      <NA>    136066    636121    716105    335477
4  5621    0   545547    368032    483010      <NA>    311054      <NA>
5  1644    0     <NA>      <NA>    194737    646673   1087661   1398481

Could you advice what should I correct here to replace NA with mean between the identified values please? Thank you very much.

ps. I post sessioninfo() information here below, as well.

> sessionInfo() 
R version 3.2.3 (2015-12-10)
Platform: i386-w64-mingw32/i386 (32-bit)
Running under: Windows 8.1 x64 (build 9600)

locale:
[1] LC_COLLATE=English_United States.1252 
[2] LC_CTYPE=English_United States.1252   
[3] LC_MONETARY=English_United States.1252
[4] LC_NUMERIC=C                          
[5] LC_TIME=English_United States.1252    

attached base packages:
[1] tcltk     parallel  stats     graphics  grDevices utils     datasets 
[8] methods   base     

other attached packages:
[1] Mfuzz_2.30.0         DynDoc_1.48.0        widgetTools_1.48.0  
[4] e1071_1.6-7          Biobase_2.30.0       BiocGenerics_0.16.1 
[7] BiocInstaller_1.20.1

loaded via a namespace (and not attached):
[1] class_7.3-14     tools_3.2.3      tkWidgets_1.48.0

You seem to have the header and row names (LABEL) are part of the data. Try

PKA <- read.table(exprsFile, sep="\t", header = TRUE, row.names = 1)

An alternative would be

library("MSnbase")
x <- readMSnSet2("C:/Users/filename.txt", ecol = 2:8, sep = "\t")
x <- impute(x, "knn")

See ?impute for other imputation methods.