Hi

When I run summarizeOverlaps as follow:

se <- summarizeOverlaps(features=ebg, reads=bamfiles,
                        mode="Union",
                        singleEnd=FALSE,
                        ignore.strand=TRUE,
                        fragments=TRUE)

It return message:"Error in .local(features, reads, mode, algorithm, ignore.strand, ...) :
  duplicate 'names(reads)' not allowed"

I wonder whether this was caused by multihits of read in Bam file?

Thanks.

See this answer to a related question. I found this other question by pasting the error into the search bar above.

Error when counting reads in genes with summarizeOverlaps (Genomic-Aligments package)