hi,

nice to meet to you, i am very new to bioconductor, and now i am trying to download data from NCBI database with getGEO

for instance, i tryed to get GSE22216.

a5<-getGEO("GSE22216")

but this will fail to get data with error as below.

ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE22nnn/GSE22216/matrix/
Error in function (type, msg, asError = TRUE)  :
  Failed to connect to ftp.ncbi.nlm.nih.gov port 21: Timed out

so, i changed the approach as below, but in this case, the next error has come out.

what should i do as a next step ? if you know it, would you teaching me ?

> a6 <- getGEO(filename="ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE22nnn/GSE22216/matrix/GSE22216_series_matrix.txt.gz")
Error in fileOpen(fname) :
  File ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE22nnn/GSE22216/matrix/GSE22216_series_matrix.txt.gz does not appear to exist.

> a7 <- getGEO(filename="ftp://130.14.250.10/geo/series/GSE22nnn/GSE22216/matrix/GSE22216_series_matrix.txt.gz")
Error in fileOpen(fname) :
  File ftp://130.14.250.10/geo/series/GSE22nnn/GSE22216/matrix/GSE22216_series_matrix.txt.gz does not appear to exist.
 

Thanks in adcance !