Dear all,

I've been using clusterProfiler for enrichment analysis of my illumina microarray data. When I try to use the function enrichKEGG I am facing following error. Any one has encountered this error. What might be the problem.

kk=enrichKEGG(gene=genes, organism="human", pvalueCutoff=0.05, pAdjustMethod="BH", qvalueCutoff=0.1, readable=FALSE)
Error in .getUrl(url, .listParser, nameColumn = 1, valueColumn = 2) : 
  invalid request, server returned Success: (200) OK (http://rest.kegg.jp/link/hsa/pathway)

sessioninfo()

R version 3.2.3 (2015-12-10)
Platform: x86_64-pc-linux-gnu (64-bit)
Running under: Ubuntu 14.04.3 LTS

locale:
 [1] LC_CTYPE=en_IN          LC_NUMERIC=C            LC_TIME=en_IN          
 [4] LC_COLLATE=en_IN        LC_MONETARY=en_IN       LC_MESSAGES=en_IN      
 [7] LC_PAPER=en_IN          LC_NAME=en_IN           LC_ADDRESS=en_IN       
[10] LC_TELEPHONE=en_IN      LC_MEASUREMENT=en_IN    LC_IDENTIFICATION=en_IN

attached base packages:
[1] parallel  stats4    stats     graphics  grDevices utils     datasets 
[8] methods   base     

other attached packages:
 [1] RDAVIDWebService_1.6.0    ggplot2_2.0.0            
 [3] GOstats_2.34.0            Category_2.34.2          
 [5] Matrix_1.2-3              KEGGREST_1.8.1           
 [7] GO.db_3.1.2               clusterProfiler_2.2.7    
 [9] hgu133plus2.db_3.1.3      SPIA_2.20.0              
[11] KEGGgraph_1.26.0          graph_1.46.0             
[13] XML_3.98-1.3              illuminaHumanv4.db_1.26.0
[15] org.Hs.eg.db_3.1.2        RSQLite_1.0.0            
[17] DBI_0.3.1                 AnnotationDbi_1.30.1     
[19] GenomeInfoDb_1.4.3        IRanges_2.2.9            
[21] S4Vectors_0.6.6           Biobase_2.28.0           
[23] BiocGenerics_0.14.0       limma_3.24.15            

loaded via a namespace (and not attached):
 [1] qvalue_2.0.0           genefilter_1.50.0      reshape2_1.4.1        
 [4] splines_3.2.3          rJava_0.9-8            lattice_0.20-33       
 [7] colorspace_1.2-6       RBGL_1.44.0            survival_2.38-3       
[10] plyr_1.8.3             KEGG.db_3.1.2          stringr_1.0.0         
[13] zlibbioc_1.14.0        Biostrings_2.36.4      munsell_0.4.2         
[16] GOSemSim_1.26.0        gtable_0.1.2           labeling_0.3          
[19] BiocInstaller_1.18.5   curl_0.9.5             GSEABase_1.30.2       
[22] Rcpp_0.12.3            xtable_1.8-2           scales_0.3.0          
[25] DO.db_2.9              annotate_1.46.1        XVector_0.8.0         
[28] png_0.1-7              stringi_1.0-1          grid_3.2.3            
[31] DOSE_2.6.6             tools_3.2.3            magrittr_1.5          
[34] httr_1.1.0             AnnotationForge_1.10.1 R6_2.1.2              
[37] igraph_1.0.1    

Output of BiocInstaller::biocValid() : 15 packages are outdated. (I am updating those)

But the data provided with package was running properly. My data is also in same format, I am not understanding where the problem is.

I will provide more details, if required.

Thank you.

The problem is that the KEGG server changed the format of thereturn value, and KEGGREST is failing to detect that the return value is actually OK. KEGGREST has been updated in the release and devel versions of Bioconductor, but you're using an older version (KEGGREST is at version 1.10.1 in the current release, note the '10' versus your '8'; likewise BiocInstaller [which determines the version of Bioconductor you are using] is at version 1.18.5 versus 1.20.1 in release, as seen on the always-release package landing page https://bioconductor.org/packages/release/BiocInstaller.

To fix this, you'll need to upgrade Bioconductor. For your version of R, this means

BiocInstaller::biocLite("BiocUpgrade")

It might be 'painful' in the sense that all Bioconductor packages will be updated, so make sure it is not immediately before thesis defense / grant deadline / etc.