How to use cdf version20 with oligo package
1
0
Entering edit mode
sylvia ▴ 10
@sylvia-5630
Last seen 6.8 years ago

HI

Sorry if this is obvious, but I was wondering how do people use custom cdf with oligo package ?

I downloaded the cdf version 20 from column 'O' for the hugene20 st array (pd.hugene20st.hs.entrezg_20.0.0.tar.gz) and installed the package and I tried to do something like this:

dat <- read.celfiles(filenames = list.celfiles(), pkgname = "pd.hugene20st.hs.entrezg")

and when I do:
> class(dat)
[1] "GenericFeatureSet"
attr(,"package")
[1] "oligoClasses"

The class of dat is GenericFeatureSet instead of  GeneFeatureSet which can not be use by rma pre-processing later on. Do people convert the GenericFeatureSet to GeneFeatureSet? or am I missing any steps when using the cdf file?

Thanks!

oligo cdf • 1.9k views
ADD COMMENT
0
Entering edit mode
@james-w-macdonald-5106
Last seen 1 day ago
United States

Why do you think that GenericFeatureSet cannot be used by rma?

> showMethods(rma)
Function: rma (package oligo)
object="ExonFeatureSet"
object="ExpressionFeatureSet"
object="GeneFeatureSet"
object="GenericFeatureSet"
object="HTAFeatureSet"
object="SnpCnvFeatureSet"

> sessionInfo()
R version 3.2.2 (2015-08-14)
Platform: x86_64-w64-mingw32/x64 (64-bit)
Running under: Windows 8 x64 (build 9200)

locale:
[1] LC_COLLATE=English_United States.1252
[2] LC_CTYPE=English_United States.1252   
[3] LC_MONETARY=English_United States.1252
[4] LC_NUMERIC=C                          
[5] LC_TIME=English_United States.1252    

attached base packages:
[1] stats4    parallel  stats     graphics  grDevices utils     datasets
[8] methods   base     

other attached packages:
[1] oligo_1.34.2        Biostrings_2.38.3   XVector_0.10.0     
[4] IRanges_2.4.6       S4Vectors_0.8.7     Biobase_2.30.0     
[7] oligoClasses_1.32.0 BiocGenerics_0.16.1

loaded via a namespace (and not attached):
 [1] affxparser_1.42.0          splines_3.2.2             
 [3] GenomicRanges_1.22.3       zlibbioc_1.16.0           
 [5] bit_1.1-12                 foreach_1.4.3             
 [7] GenomeInfoDb_1.6.2         tools_3.2.2               
 [9] SummarizedExperiment_1.0.2 ff_2.2-13                 
[11] DBI_0.3.1                  iterators_1.0.8           
[13] preprocessCore_1.32.0      affyio_1.40.0             
[15] codetools_0.2-14           RSQLite_1.0.0             
[17] compiler_3.2.2             BiocInstaller_1.20.1   
ADD COMMENT
0
Entering edit mode

Hi James,

I might have load affy package at the same time and didn't specify which rma to use and when I read the arguments for rma from oligo package the object is : Exon/HTA/Expression/Gene/SnpCnv-FeatureSet so I thought it doesn't take GenericFeatureSet! Sorry for the confusion, now it works!

Thanks!

ADD REPLY

Login before adding your answer.

Traffic: 644 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6