HI
Sorry if this is obvious, but I was wondering how do people use custom cdf with oligo package ?
I downloaded the cdf version 20 from column 'O' for the hugene20 st array (pd.hugene20st.hs.entrezg_20.0.0.tar.gz) and installed the package and I tried to do something like this:
dat <- read.celfiles(filenames = list.celfiles(), pkgname = "pd.hugene20st.hs.entrezg")
and when I do:
> class(dat)
[1] "GenericFeatureSet"
attr(,"package")
[1] "oligoClasses"
The class of dat is GenericFeatureSet instead of GeneFeatureSet which can not be use by rma pre-processing later on. Do people convert the GenericFeatureSet to GeneFeatureSet? or am I missing any steps when using the cdf file?
Thanks!
Hi James,
I might have load affy package at the same time and didn't specify which rma to use and when I read the arguments for rma from oligo package the object is : Exon/HTA/Expression/Gene/SnpCnv-FeatureSet so I thought it doesn't take GenericFeatureSet! Sorry for the confusion, now it works!
Thanks!