Hello, to speed up learning to use bioconductor tools, I would like to see a simple example on writing out data from a fcs file and to a simple text file (maybe only the first 1000 events/measurements). I can use flowViz to plot and flowStats to make analysis, but I would like to use for example my own program or linux/gnuplot to confirm I have understood.
Given the example below (the fcs-file is here calles "rkut1.fcs"), how I print out FITC-A values to a text file (one value each line):
> library(flowCore)
> x <- read.FCS("rkut1.fcs", transformation=FALSE)
> x
flowFrame object 'rkut1.fcs'
with 54289 cells and 10 observables:
name desc range minRange maxRange
$P1 HDR-T HDR-T 128 0.0000000 127
$P2 FSC-A FSC-A 1024 0.0000000 1023
$P3 SSC-A SSC-A 1024 0.0000000 1023
$P4 V1-A VioBlue-A 1024 -0.7600108 1023
$P5 B1-A FITC-A 1024 -0.3138263 1023
$P6 B2-A PE-A 1024 -0.3507906 1023
$P7 B3-A PI/PE-Cy5.5-A 1024 -0.3008470 1023
$P8 B4-A PE-Cy7-A 1024 -0.4390466 1023
$P9 R1-A APC-A 1024 -0.3781420 1023
$P10 R2-A APC-Cy7-A 1024 -1.3673245 1023
144 keywords are stored in the 'description' slot
>
Thanks in advance, Reinert
If you'd like to attract help from the flowCore authors, enter 'flowCore' as a tag for your question.