I was running DESeq2. However after running DESEq() I got the following:
> dds<-DESeq(dds);
using pre-existing size factors
estimating dispersions
gene-wise dispersion estimates
Error in .Call2("solve_user_SEW0", start, end, width, PACKAGE = "IRanges") :
solving row 463827: negative widths are not allowed
The sessionInfo is given below:
> sessionInfo()
R version 3.2.3 (2015-12-10)
Platform: x86_64-apple-darwin13.4.0 (64-bit)
Running under: OS X 10.9.2 (Mavericks)
locale:
[1] en_GB.UTF-8/en_GB.UTF-8/en_GB.UTF-8/C/en_GB.UTF-8/en_GB.UTF-8
attached base packages:
[1] parallel stats4 stats graphics grDevices utils datasets methods base
other attached packages:
[1] DESeq2_1.10.0 RcppArmadillo_0.6.300.2.0 Rcpp_0.12.2 SummarizedExperiment_1.0.1
[5] Biobase_2.30.0 GenomicRanges_1.22.2 GenomeInfoDb_1.6.1 IRanges_2.4.6
[9] S4Vectors_0.8.5 BiocGenerics_0.16.1 BiocInstaller_1.20.1
loaded via a namespace (and not attached):
[1] RColorBrewer_1.1-2 futile.logger_1.4.1 plyr_1.8.3 XVector_0.10.0 futile.options_1.0.0 tools_3.2.3
[7] zlibbioc_1.16.0 rpart_4.1-10 digest_0.6.8 RSQLite_1.0.0 annotate_1.48.0 gtable_0.1.2
[13] lattice_0.20-33 DBI_0.3.1 proto_0.3-10 gridExtra_2.0.0 genefilter_1.52.0 cluster_2.0.3
[19] stringr_1.0.0 locfit_1.5-9.1 nnet_7.3-11 grid_3.2.3 AnnotationDbi_1.32.2 XML_3.98-1.3
[25] survival_2.38-3 BiocParallel_1.4.0 foreign_0.8-66 latticeExtra_0.6-26 Formula_1.2-1 geneplotter_1.48.0
[31] ggplot2_1.0.1 reshape2_1.4.1 lambda.r_1.1.7 magrittr_1.5 scales_0.3.0 Hmisc_3.17-0
[37] MASS_7.3-45 splines_3.2.3 xtable_1.8-0 colorspace_1.2-6 stringi_1.0-1 acepack_1.3-3.3
[43] munsell_0.4.2
Please advice me. Thank you in advance.
