Hello,

I am trying to create a qPCR set using the R package HTqPCR. My input file is a matrix of CT values for 254 miRNAs from 27 samples ( divided in 3 groups).

How do I use the readCtData function to create qPCR set for my input file?

Thanks

Sharvari

Alternatively you could input the matrix of Ct values directly into limma, then there's no need for a qPCR set. See for example Performing differential gene analysis on RT PCR experimental data