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SteffenMöller
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30
@steffenmoller-713
Last seen 10.2 years ago
Hello,
I just made my first experiences with affylmGUI and am very excited
about it. Many thanks for this!
Some questions/comments rather important to me:
* With three different targets, say A (complete vaccine),B (only the
adjuvant), C (untreated)
I am only allowed to enter two contrasts. This is probably
mathematically fine since the third is not linearly independent of
the first two, however, for drawing the VennDiagram and the HeatMap,
I am very iterested to see
A-B, A-C and B-C
which make it three contrasts that apparently I cannot specify in
affylmgui. What should I do?
* Can I combine target specifications? E.g. A (sick) vs B+C (healthy),
referring to the A,B,C of above?
* When plotting a heatmap, it is always the first contrast that is
used
as a reference, whatever I contrast I specify in the window.
Some less important issues
* I can extend the window for the TopTable, but the table itself is
not
extended.
* The font size of the Heatmap is not changing with a resize of the
drawing,
* When selecting gcrma but not having the library gcrma installed,
lots
wish errors pop up, but affylmGUI lists the normalisation as
available.
* For the report I suggest to help the authors of the paper with a
methods section that lists references for the methods used,
parameters
and the like. Maybe I can come up with a suggestive patch for
this. GeneSpring has this feature and I always found this a bit
funny -
it isn't. Amazon would have patented this :-) Btw, the report is
great!
* Consider to have a window with a log of the R statements required to
achieve a particular result.
Many greetings and thanks in advance for any help
Steffen
--
Steffen Moeller, PhD
University of Rostock
Institute of Immunology
moeller@pzr.uni-rostock.de