Dear list memebers, I was looking at affycomp benchmark results and found some entries that I do not understand. Can anyone tell me what '+32' and '+16' mean in MAS+32 and PLIER+16? I think they have to do with variance stabilization but I would appreciate if anyone could give my further details. In addition, can anyone point me to a technical reference regarding the Affymetrix new PLIER summarization method? Is it a whole solution, i.e. background substraction + probe level normalization + probeset expression estimation, or just the latter? In that case which background and normalization steps are advice to be used with? Thanks, Ariel Chernomoretz, Ph.D. Centre de recherche du CHUL 2705 Blv Laurier, bloc T-367 Sainte-Foy, Qc G1V 4G2 (418)-525-4444 ext 46339