non-symmetric heatmap colors
3
0
Entering edit mode
@arnemullersanofi-aventiscom-1086
Last seen 10.2 years ago
Hello, well, it's about heatmap colors, again ... . Does anybody know a package to create a non-symmetric color map with user-choosen mir point and satuaration? I've a fold-change matrix, with values from -3 fold (ratio 1/3) to +7 fold. I'd like to go from 0.5 (green) to 1 (black) to 3 (red). I think maPallete just assigns the lowest FC to the first color and the highest FC to the last color, i.e. an evenly distributed color list, right? Any hints are welcome, Arne
GO GO • 2.2k views
0
Entering edit mode
@sean-davis-490
Last seen 3 months ago
United States
Look at using the breaks argument. If you do breaks=seq(-3,3,0.1) and use 59 colors (this is important because the number of breaks and colors have to match--see documentation for heatmap), you get "black" centered on zero and red and green symmetrically set around zero (if using red/green colors, obviously). Sean On Feb 2, 2005, at 12:10 PM, <arne.muller@sanofi-aventis.com> wrote: > Hello, > > well, it's about heatmap colors, again ... . > > Does anybody know a package to create a non-symmetric color map with > user-choosen mir point and satuaration? I've a fold-change matrix, > with values from -3 fold (ratio 1/3) to +7 fold. I'd like to go from > 0.5 (green) to 1 (black) to 3 (red). > > I think maPallete just assigns the lowest FC to the first color and > the highest FC to the last color, i.e. an evenly distributed color > list, right? > > Any hints are welcome, > > Arne > > _______________________________________________ > Bioconductor mailing list > Bioconductor@stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor
ADD COMMENT
0
Entering edit mode
@wolfgang-huber-3550
Last seen 3 months ago
EMBL European Molecular Biology Laborat…
Hi Arne, try the following: library(RColorBrewer) library(geneplotter) f = colorRamp(brewer.pal(11, "RdBu")) then f is a functions that takes numbers between 0 and 1 and maps them to colors between red and blue. And g = function(x) f( (x-x0)/(x1-x0) ) maps numbers between x0 and x1 to these colors. Best wishes Wolfgang Arne.Muller@sanofi-aventis.com wrote: > Hello, > > well, it's about heatmap colors, again ... . > > Does anybody know a package to create a non-symmetric color map with user-choosen mir point and satuaration? I've a fold-change matrix, with values from -3 fold (ratio 1/3) to +7 fold. I'd like to go from 0.5 (green) to 1 (black) to 3 (red). > > I think maPallete just assigns the lowest FC to the first color and the highest FC to the last color, i.e. an evenly distributed color list, right? > > Any hints are welcome, > > Arne ------------------------------------- Wolfgang Huber European Bioinformatics Institute European Molecular Biology Laboratory Cambridge CB10 1SD England Phone: +44 1223 494642 Fax: +44 1223 494486 Http: www.ebi.ac.uk/huber
ADD COMMENT
0
Entering edit mode
@arnemullersanofi-aventiscom-1086
Last seen 10.2 years ago
Wolfgang and Sean, thanks for your hints. The "breaks" argument (described in ?image), did what I wanted. I just had to set all fold change values < -3 to -3 and > 3 to 3 to get a saturation at these limits, otherwise the color beyond these "extreme" values would just be white. kind regards, Arne > -----Original Message----- > From: bioconductor-bounces@stat.math.ethz.ch > [mailto:bioconductor-bounces@stat.math.ethz.ch]On Behalf Of Wolfgang > Huber > Sent: 03 February 2005 04:49 > Cc: bioconductor@stat.math.ethz.ch > Subject: Re: [BioC] non-symmetric heatmap colors > > > Hi Arne, > > try the following: > > library(RColorBrewer) > library(geneplotter) > f = colorRamp(brewer.pal(11, "RdBu")) > > then f is a functions that takes numbers between 0 and 1 and maps them > to colors between red and blue. > > And > > g = function(x) f( (x-x0)/(x1-x0) ) > > maps numbers between x0 and x1 to these colors. > > Best wishes > Wolfgang > > > Arne.Muller@sanofi-aventis.com wrote: > > Hello, > > > > well, it's about heatmap colors, again ... . > > > > Does anybody know a package to create a non-symmetric color > map with user-choosen mir point and satuaration? I've a > fold-change matrix, with values from -3 fold (ratio 1/3) to > +7 fold. I'd like to go from 0.5 (green) to 1 (black) to 3 (red). > > > > I think maPallete just assigns the lowest FC to the first > color and the highest FC to the last color, i.e. an evenly > distributed color list, right? > > > > Any hints are welcome, > > > > Arne > > ------------------------------------- > Wolfgang Huber > European Bioinformatics Institute > European Molecular Biology Laboratory > Cambridge CB10 1SD > England > Phone: +44 1223 494642 > Fax: +44 1223 494486 > Http: www.ebi.ac.uk/huber > > _______________________________________________ > Bioconductor mailing list > Bioconductor@stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor >
0
Entering edit mode
On Feb 3, 2005, at 4:30 AM, <arne.muller@sanofi-aventis.com> wrote: > Wolfgang and Sean, > > thanks for your hints. The "breaks" argument (described in ?image), > did what I wanted. I just had to set all fold change values < -3 to > -3 and > 3 to 3 to get a saturation at these limits, otherwise the > color beyond these "extreme" values would just be white. > If you use the heatmap.2 function in the gplots package (part of the gregmisc bundle), it deals with the saturation issue automatically and assigns all values above (below) the top (bottom) break to the most extreme color--no more white! It also adds some other very nice bells-and-whistles to the standard heatmap function. I now use heatmap.2 exclusively. Sean
ADD REPLY

Login before adding your answer.

Traffic: 525 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6