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Auer Michael
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250
@auer-michael-953
Last seen 10.2 years ago
Hi
I am starting to work with cDNA chips from array comparative
hybridization. I have severel arrays image analysed with spot 2.0, but
the
parameters (Excel Columns reported) are quite different from the ones
in
swirl.spot in the marray package, which has the command read.Spot.
There
are the following parameters in the Excel sheet. Can anybody help me
further on how to read them in in a proper way? I would like to
normalize
them in different ways, but first I need to have the two channels in
R.
Furthermore the genes were spotted in triplicates. Is there a way to
tell
that in marray or any other package too?
Thank you very much
Arr-rowy
Spot-colx
Spot-rowy
Flag
nfore
nback
DapiFore
DapiBack
Dapi
TestFore
TestBack
Test
RefFore
RefBack
Ref
Log2Rat
RawRat
SpotCorr
MinF-B
Slope
Log2Slope
TestIntercept
RefIntercept
OriginDist
MeanLog2Rat
MeanRat
MedianRatio
Log2MedRat