mas5, gcrma, rma
0
0
Entering edit mode
Auer Michael ▴ 250
@auer-michael-953
Last seen 10.2 years ago
I have a few genes, where rtPCR has been done. I have normalized the arrays with mas5, rma and gcrma. Here are the Fold Changes of the genes. GCRMA shows the biggest correlation with pcr. Still I cannot figure out any systematics except that gcrma shows smaller values. If a cut off fold change of 1.5 is taken, only 3 genes are being selected. The distributions of the normalized signals look very different. GCRMA shows a bimodal distribution. Furthermore, does anybody know if there exists smth like a change p value for rma or gcrma, like in the mas5 software? Thanks a lot for your help mas5 pcr rma gcrma BTG2 -1,5 -1,5 -1,6 -1,3 EFNB2 -1,6 -1,4 -1,6 -1,4 EPAS1 -1,8 -1,4 -3,1 -1,6 EZH1 -2,8 -1,5 -1,2 -1,1 FN1 -1,9 -1,4 -2 -1,7 FOXF2 -1,5 -1,4 -1,1 -1,1 MMP14 -1,9 -2,0 -2 -1,6 PTBP1 -2,2 -1,3 -1,9 -1,4 VIL2 -1,4 -1,6 -1,5 -1,3
gcrma gcrma • 912 views
ADD COMMENT

Login before adding your answer.

Traffic: 573 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6