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Auer Michael
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@auer-michael-953
Last seen 10.2 years ago
I have a few genes, where rtPCR has been done. I have normalized the
arrays with mas5, rma and gcrma. Here are the Fold Changes of the
genes.
GCRMA shows the biggest correlation with pcr. Still I cannot figure
out
any systematics except that gcrma shows smaller values. If a cut off
fold
change of 1.5 is taken, only 3 genes are being selected. The
distributions
of the normalized signals look very different. GCRMA shows a bimodal
distribution.
Furthermore, does anybody know if there exists smth like a change p
value
for rma or gcrma, like in the mas5 software?
Thanks a lot for your help
mas5 pcr rma gcrma
BTG2 -1,5 -1,5 -1,6 -1,3
EFNB2 -1,6 -1,4 -1,6 -1,4
EPAS1 -1,8 -1,4 -3,1 -1,6
EZH1 -2,8 -1,5 -1,2 -1,1
FN1 -1,9 -1,4 -2 -1,7
FOXF2 -1,5 -1,4 -1,1 -1,1
MMP14 -1,9 -2,0 -2 -1,6
PTBP1 -2,2 -1,3 -1,9 -1,4
VIL2 -1,4 -1,6 -1,5 -1,3