Differential Expression of c.diff. microarray
2
0
Entering edit mode
SSK ▴ 10
@ssk-7679
Last seen 9.6 years ago
United Kingdom

Hello,

I am interested in determining the differential expression in E-GEOD-37442. Does anyone know of some code that I can adapt to the analysis of this array. In the array description it says A-GEOD-15470 - Agilent-024835 CDv2_0_1 [ProbeName version] 

Thank you so much!

arrayexpress differential expression • 1.5k views
ADD COMMENT
0
Entering edit mode
@james-w-macdonald-5106
Last seen 1 day ago
United States

Sure. There is a metric ton of code in the limma User's guide that you could adapt.

ADD COMMENT
0
Entering edit mode
SSK ▴ 10
@ssk-7679
Last seen 9.6 years ago
United Kingdom

 

I have read the manual for Limma but I am not sure of the specifications for E-GEOD-37442. I have the following line of code:

SDRF <- read.delim("E-GEOD-37442.sdrf.txt",check.names=FALSE,stringsAsFactors=FALSE)

but i am not sure what are the specifications for read.maimages? I have this line for the Limma manual but I am not sure how I can adapt it for E-GEOD-37442.

x <- read.maimages(SDRF[,"Array Data File"],source="agilent",green.only=TRUE)

Also, would you know if there are microarray analysis services provided somewhere? I am worried I can't learn all of the tools within a reasonable time frame.

 

ADD COMMENT
0
Entering edit mode

Yes. There are microarray analysis services provided lots of places. Did you try a google search?

ADD REPLY

Login before adding your answer.

Traffic: 550 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6