I used gage in order to perform a pathway enrichment analysis, using a table with KO numbers. Œs it possible to retrieve information about the abundance of the differential pathways? Because I have only KO numbers in my table (without all the kegg identification levels), so I don't know how to collapse them to pathways and find out how abundant is a specific pathway in each sample.
If you want the summary expression perturbation for each pathway in each sample, you can check the stats element of the gage analysis results. Following the example in the main gage tutorial, you can do:
…
> gse16873.kegg.p <- gage(gse16873, gsets = kegg.gs,
+ ref = hn, samp = dcis)
> names(gse16873.kegg.p)
[1] "greater" "less" "stats"
> head(gse16873.kegg.p$stats)
…(a matrix with pathway as rows and samples as columns)
> str(gse16873.kegg.p$stats)
…
HTHs,
Weijun
If you want the summary expression perturbation for each pathway in each sample, you can check the stats element of the gage analysis results. Following the example in the main gage tutorial, you can do:
…
> gse16873.kegg.p <- gage(gse16873, gsets = kegg.gs,
+ ref = hn, samp = dcis)
> names(gse16873.kegg.p)
[1] "greater" "less" "stats"
> head(gse16873.kegg.p$stats)
…(a matrix with pathway as rows and samples as columns)
> str(gse16873.kegg.p$stats)
…
HTHs,
Weijun
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On Fri, 2/27/15, francesca.defilippis [bioc] <noreply@bioconductor.org> wrote:
Subject: [bioc] retrieve kegg pathway abundances from gage analysis
To: luo_weijun@yahoo.com
Date: Friday, February 27, 2015, 11:32 AM
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User francesca.defilippis
wrote Question:
retrieve kegg pathway abundances from gage analysis:
Hi!
I used gage in order to perform a pathway enrichment
analysis, using a table with KO numbers. Œs it possible to
retrieve information about the abundance of the differential
pathways? Because I have only KO numbers in my table
(without all the kegg identification levels), so I don't
know how to collapse them to pathways and find out how
abundant is a specific pathway in each sample.
Thanks
Francesca
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retrieve kegg pathway abundances from gage analysis