Question

riboSeqR: criteria used to determine reading frames

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antonvila.s • 0

@antonvilas-7285

Last seen 9.1 years ago

Spain

Hi,

I'm having trouble to understand which criteria are used for the determination of the reading frame in roboSeqR. Is it the position of the 5' nucleotide relative to the START codon, the position of the first base of the A-site codon, other?

best,

Antón

riboSeqR • 1.3k views

ADD COMMENT • link updated 9.7 years ago by Thomas J Hardcastle ▴ 180 • written 9.7 years ago by antonvila.s • 0

score 0 · Answer 1 · 2015-03-02

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Thomas J Hardcastle ▴ 180

@thomas-j-hardcastle-3860

Last seen 7.1 years ago

United Kingdom

A read that starts in frame with the A of the ATG will be in frame 0, that which starts in frame with the T will be in frame 1, et cetera.

ADD COMMENT • link 9.7 years ago Thomas J Hardcastle ▴ 180