CDF files
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@srinivas-iyyer-939
Last seen 10.2 years ago
Hi, CellHeader=X Y MEAN STDV NPIXELS 0 0 152.0 30.0 25 1 0 5024.5 475.3 20 2 0 151.0 25.0 25 3 0 5017.0 558.9 25 4 0 205.5 71.5 20 How can I get to know what 0 0 , 1 0, 2 0 correspond to 1001_at or XXXX_at. Can some one please help. I want to get the spot and the corresponding probe. Thank you. Srini
probe probe • 905 views
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@lgautieralternorg-747
Last seen 10.2 years ago
Srinivas Iyyer wrote: > Hi, > > CellHeader=X Y MEAN STDV NPIXELS > 0 0 152.0 30.0 25 > 1 0 5024.5 475.3 20 > 2 0 151.0 25.0 25 > 3 0 5017.0 558.9 25 > 4 0 205.5 71.5 20 > > > > > > How can I get to know what 0 0 , 1 0, 2 0 correspond > to 1001_at or XXXX_at. Can some one please help. I > want to get the spot and the corresponding probe. The information is contained in the relevant CDF file. Did you consider using the 'affy' package ? The help page for the function 'xy2indices' might be worth a look. However, if you want to go from XY coordinates to probe set names often in your code, the most efficient would be to use the class Cdf (now found in the package 'makecdfenv'). L. > Thank you. > > Srini > > _______________________________________________ > Bioconductor mailing list > Bioconductor@stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor >
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Hi, I am also trying to parse CDF files to get the coordinates of the porbesets and their names. The reason I need to get is that, I want a reference for each probeset on the chip in this case postion on the chip. This allows my to query specifically some probesets depending on the position. I know this might sound too much, however, I am interested in implementing this in the db schema I have. I tried Laurent's suggestions. It worked for one probeset. Is there any command where I get an object with all probesetIDs and their coordinates written adjascent to probeset coordinats. Thanks. Peri. --- Laurent Gautier <lgautier@altern.org> wrote: > Srinivas Iyyer wrote: > > Hi, > > > > CellHeader=X Y MEAN STDV NPIXELS > > 0 0 152.0 30.0 25 > > 1 0 5024.5 475.3 20 > > 2 0 151.0 25.0 25 > > 3 0 5017.0 558.9 25 > > 4 0 205.5 71.5 20 > > > > > > > > > > > > How can I get to know what 0 0 , 1 0, 2 0 > correspond > > to 1001_at or XXXX_at. Can some one please help. > I > > want to get the spot and the corresponding probe. > > The information is contained in the relevant CDF > file. > > Did you consider using the 'affy' package ? > > The help page for the function 'xy2indices' might be > worth a look. > > However, if you want to go from XY coordinates to > probe set names often > in your code, the most efficient > would be to use the class Cdf (now found in the > package 'makecdfenv'). > > > L. > > > > Thank you. > > > > Srini > > > > _______________________________________________ > > Bioconductor mailing list > > Bioconductor@stat.math.ethz.ch > > https://stat.ethz.ch/mailman/listinfo/bioconductor > > > > _______________________________________________ > Bioconductor mailing list > Bioconductor@stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor >
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S Peri wrote: > Hi, > I am also trying to parse CDF files to get the > coordinates of the porbesets and their names. The > reason I need to get is that, I want a reference for > each probeset on the chip in this case postion on the > chip. This allows my to query specifically some > probesets depending on the position. I know this > might sound too much, however, I am interested in > implementing this in the db schema I have. > > I tried Laurent's suggestions. It worked for one > probeset. By induction, it should then work for all probesets. (and since apparently you want to store everything in a database, I do not see the problem...) > Is there any command where I get an object > with all probesetIDs and their coordinates written > adjascent to probeset coordinats. Follow the second suggestion and make an instance of class Cdf (see package 'makecdfenv'). Then: i <- seq(along=c(mycdf@names)) cbind(mycdf@names.levels[mycdf@names], indices2xy(i, nr=nrow(mycdf@names)) ## ...this is untested... you may still have to read the ## documentation... > Thanks. > Peri. > > > --- Laurent Gautier <lgautier@altern.org> wrote: > > >>Srinivas Iyyer wrote: >> >>>Hi, >>> >>>CellHeader=X Y MEAN STDV NPIXELS >>> 0 0 152.0 30.0 25 >>> 1 0 5024.5 475.3 20 >>> 2 0 151.0 25.0 25 >>> 3 0 5017.0 558.9 25 >>> 4 0 205.5 71.5 20 >>> >>> >>> >>> >>> >>>How can I get to know what 0 0 , 1 0, 2 0 >> >>correspond >> >>>to 1001_at or XXXX_at. Can some one please help. >> >>I >> >>>want to get the spot and the corresponding probe. >> >>The information is contained in the relevant CDF >>file. >> >>Did you consider using the 'affy' package ? >> >>The help page for the function 'xy2indices' might be >>worth a look. >> >>However, if you want to go from XY coordinates to >>probe set names often >>in your code, the most efficient >>would be to use the class Cdf (now found in the >>package 'makecdfenv'). >> >> >>L. >> >> >> >>>Thank you. >>> >>>Srini >>> >>>_______________________________________________ >>>Bioconductor mailing list >>>Bioconductor@stat.math.ethz.ch >>>https://stat.ethz.ch/mailman/listinfo/bioconductor >>> >> >>_______________________________________________ >>Bioconductor mailing list >>Bioconductor@stat.math.ethz.ch >>https://stat.ethz.ch/mailman/listinfo/bioconductor >> > > > _______________________________________________ > Bioconductor mailing list > Bioconductor@stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor >
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