Fail to read qcFile in readBeadSummaryData function
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junjeong • 0
@junjeong-7244
Last seen 9.9 years ago
Korea, Republic Of

Hi, i am trying to use beadarray package for my illumina expression data.  Thing is whenever I tried readBeadSummaryData function with codes below, just to find empty QC information likie below.

My qcFile is controlprobeprofile.txt acquired from genomestudio. Is there anybody that has similar problem and solution? Thanks in advance!!

 

 

Data=readBeadSummaryData(dataFile, qcFile=qcFile, sampleSheet=NULL,
                           sep="\t", skip=0, ProbeID="Probe_ID",
                           columns = list(exprs = "AVG_Signal", se.exprs="BEAD_STDERR",
                                          nObservations = "Avg_NBEADS", Detection="Detection Pval"),
                           qc.sep="\t", qc.skip=0, controlID="TargetID",
                           qc.columns = list(exprs="AVG_Signal", se.exprs="BEAD_STDERR",
                                             nObservations="Avg_NBEADS", Detection="Detection Pval"),
                           illuminaAnnotation="Humanv3", dec=".", quote="", annoCols = c("TargetID", "PROBE_ID","SYMBOL"))

 

ExpressionSetIllumina (storageMode: list)
assayData: 13447 features, 5 samples 
  element names: exprs, se.exprs, nObservations, Detection 
protocolData: none
phenoData
  rowNames: 9683758072_E 9683758071_J ...
    9999593135_G (5 total)
  varLabels: sampleID
  varMetadata: labelDescription
featureData
  featureNames: 60689 1580504 ... NEGATIVE (13447
    total)
  fvarLabels: ProbeID TargetID ... Status (5 total)
  fvarMetadata: labelDescription
experimentData: use 'experimentData(object)'
Annotation: Humanv3 
QC Information
 Available Slots:  
  QC Items: 
  sampleNames:
beadarray • 1.4k views
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Entering edit mode
meeta.mistry ▴ 30
@meetamistry-7355
Last seen 2.4 years ago
United States

Hi, I am having a similar problem. Any chance you have an answer to this? Thanks,

 

Meeta

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