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kvyas
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@kvyas-940
Last seen 10.2 years ago
I have made some progress.
Change dir(to where CEL files are saved)
Data <- ReadAffy()
eset <- rma(Data)
my.pm.data <- pm(Data)
write.table (my.pm.data, file = "pmprobes3.csv", sep = ",", col.names
= TRUE,
row.names = probeNames(Data))
This results in the following:
Probe Sam1 Sam2 Sam3 Sam4
1415670_at01 340 383 376 320
1415670_at02 794 932 734 697
1415670_at03 1117 1366 1013 932
1415670_at04 97 97 148 94
1415670_at05 387 405 367 402
1415670_at06 485 553 391 343
1415670_at07 458 509 410 385
1415670_at08 507 684 616 545
1415670_at09 311 392 576 463
1415670_at10 691 822 688 588
1415670_at11 512 726 3201 2317
1415671_at01 1230 1212 873 770
1415671_at02 886 877 794 729
1415671_at03 1472 1682 1263 1152
1415671_at04 1171 1214 948 914
1415671_at05 390 479 435 396
1415671_at06 462 513 423 389
1415671_at07 987 1151 1102 815
1415671_at08 992 1066 1203 1013
1415671_at09 489 534 501 385
1415671_at10 401 509 379 293
1415671_at11 1443 1686 1410 1215
How can I get the p-value for each probe?
>===== Original Message From Frank Duan <fhduan@gmail.com> =====
>use PM function in Affy package.
>
>Frank
>
>
>On Mon, 4 Oct 2004 16:48:12 -0400, kvyas <kvyas@utk.edu> wrote:
>> I have CEL files from Affymetrix Mouse Array 430_2 and am trying to
get the
>> the individual PM intensities (11 per gene) for each sample. I
would like
to
>> write out this into a tab delimited text file. Where am I stalling?
This is
>> what I've done:
>>
>> Change dir(to where CEL files are saved)
>> Data <- ReadAffy()
>> eset <- rma(Data)
>> write.exprs(eset, file="mydata.txt")
>>
>> With this I am only able to get the average intensities per gene
not the
>> intensity per probe of each gene.
>>
>> Please help me out.
>>
>> Kanan
>>
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