Hello Everyone,

I am interested in alternative splicing patterns for genes from this data set :

http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE17395

Any Bioconductor package for analyzing the data for alternative splicing?

Also the supplementary files has .bar extension files. I assume they are processed files, but they are binary. How can I view them?

Thanks for the help,

Regards

gv

The diffSplice function in the limma package detects differential splicing using exon-level expression values from exon microarrays or from RNA-seq.

Using a tiling array for the Saccharomyces cerevisiae genome is a little different, but you could perhaps group probes by putative genes and treat each probe as an exon.

Hi All

I am planning to use the diffsplice function. I could not figure out what sort of count data format does it accept. I am using featureCounts to generate the counts from BAM files.

Thanks heaps for your help!

Sonika