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Matthew Hannah
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940
@matthew-hannah-621
Last seen 10.1 years ago
Hi,
Quick question this time. Basically these dyes have higher intensity,
less quenching and correlate more between dye pairs. The ULYSIS kit
uses
chemical labelling which eliminates sequence effects.
http://www.probes.com/servlets/directory?id1=6&id2=48&id3=319
http://www.probes.com/media/publications/394.pdf
As far as I know Limma accounts for dye bias during normalisation. If
there is less/no bias will there be artifacts introduced? Or other
points I need to be aware of?
Thanks,
Matt