Hi all, Does anyone use Metamorph software for Image analysis and Object tracking? If yes... I have a stack of images showing fluorecent vesicles that are moving. In the image they appear as small red spots with various shapes (large and small/just 2-3 pixels). I am not sure which procedure I should follow to track a random vesicle. I have manual tracking and automated tracking. When I try manually, the results I take are very different from the automated procedure. I suspect that the automated tracking is not adequate. Any suggestions? Thanks in advance. Makis ------------------------- E Motakis, Mathematics E.Motakis@bristol.ac.uk