Hi Alejandro, You need to provide a little more detail in order for me to help. What exactly is not working as expected? Can you please provide a simple reproducible example? Please also provide the output of sessionInfo() for me to see which package and R version we are talking about. If your data are available in the form of a rectangular matrix you can simply provide that to the DataTrack() constructor and play with the ?groups? display parameter to overlay multiple sample groups on the same panel. OverlayTrack() is really meant for combining data that is not rectangular in nature, or when combining different plotting options for different samples. Florian From: Alejandro Berr?o <alebesc@gmail.com> Date: Sunday 17 August 2014 19:31 To: Florian Hahne <florian.hahne at="" novartis.com=""> Subject: Re: Question on Gviz Hello there, I have been working with Gviz for a long time and I am very happy with it. Thanks for all your work. But I am just curious why the OverlayTrack and HighlightTrack functions are not working. I tried to update the library but it doesn't work as well. I am trying to plot two sets of data with same Y and X axis but I haven't figured out how to combine two DataTracks into 1 without using Overlaytrack function. I will appreciate any help! Thank! On Thu, Mar 27, 2014 at 3:34 AM, Hahne, Florian <florian.hahne at="" novartis.com=""> wrote: Hi Alejandro, Without seeing the structure of your data table I can?t be very concrete, but in general the Gviz package should give you all the flexibility you need in order to create a DataTrack object from it. You may want to carefully (re-)read the section about DataTracks in the package vignette, and also the documentation for the DataTrack() constructor (by typing '? DataTrack?). Hopefully this will already get you much closer to a solution. If you still feel that you are stuck after doing this home work, please send me a reduced example of your conservation table (the first couple of rows suffice) and I can help you with more concrete code suggestions. Florian ----------------------------------------------- Florian Hahne Expert Data Integration and Bioinformatics PCS Informatics Novartis Pharma AG, NIBR Klybeck Novartis Institutes for Biomed. Research WKL-135.P.18 Klybeckstrasse 141 CH-4057 Basel SWITZERLAND From: Alejandro Berr?o <alebesc@gmail.com> Date: Wednesday 26 March 2014 16:51 To: Florian Hahne <florian.hahne at="" novartis.com=""> Subject: Question on Gviz Hello Florian, I am so excited with the Gviz package. I like it a lot and I hope I can make some nice genomic graphics. I was wondering if you can help me to solve a little problem I have. Here is the thing, I am interested in plotting a track from tabular data via converting a phascons conservation table that I downloaded using galaxy as xxx.tabular which then I modified at the position column from mouse (mm9) to another species data using a MAFFT alignment. I can plot the mm9 conservation table using UcscTrack but I cant plot the modified table I have in my folder because this is not in a datatrack format. Is it possible to make the conversion? I would appreciate any help and thanks in advance! Cheers, -- Alejandro Berr?o, PhD Candidate University of Texas at Austin www.alejoberrio.com <http: www.alejoberrio.com=""> -- Alejandro Berr?o, PhD Candidate University of Texas at Austin www.alejoberrio.com <http: www.alejoberrio.com="">