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Last seen 10.3 years ago
Dear all,
this is my first time with bead array and i have been struggling with
the sampleSheet for a while now, see error message below (...directory
does not exist). I am trying to read bead level intensities from one
Human HT12 chip, without the images, using readIllumina. If i omit the
sampleSheet, BeadArray is able to read my data, so they should be OK.
I am also able to read the data from the BeadArrayUseCases - including
the sampleSheet. I have taken great care to make sure my sampleSheet-
file is identical to the one from the UseCase, but still i get the
error message reported below.
I am really frustrated and cannot understand why my file does not work
when the UseCase one does and would be really grateful for comments.
Best,
Christine
-- output of sessionInfo():
input:
NingDataSample =readIllumina(dir="/Users/femcs/Documents/Bioinfo/Bioco
nductor/NingLu/9031292034_data",
sectionNames = NULL,
useImages = FALSE,
illuminaAnnotation ='Humanv4')
output WITH sample sheet:
Sample Sheet
/Users/femcs/Documents/Bioinfo/Bioconductor/SampleSheet.csv will be
used to read the data
Error in analyseDirectory(dir = x, sectionNames =
as.character(dirs[[x]]), :
Directory does not exist.
output WITHOUT sampleSheet structure of object;
> str(NingData)
Formal class 'beadLevelData' [package "beadarray"] with 4 slots
..@ beadData :List of 12
.. ..$ 9031292034_A_perBeadFile:List of 4
.. .. ..$ ProbeID:<environment: 0x7fb615574fc8="">
.. .. ..$ GrnX :<environment: 0x7fb61555b158="">
.. .. ..$ GrnY :<environment: 0x7fb615536a30="">
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