Problem in generating heatmap after R and Bioconductor Update
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Guest User ★ 13k
@guest-user-4897
Last seen 10.3 years ago
Dear list, I'm having issues in generating an heatmap which occurred only after the upgrade to ver. 3.1.1 Below you find a very easy example which works in previous R version 3.0.2 but not in ver. 3.1.1 giving the following error. Error in heatmap.2(t(mtcars), dendrogram = c("col"), Rowv = FALSE, Colv = as.dendrogram(xClust)) : Colv dendrogram doesn't match size of x I've also added sessionInfo() for the NOT WORKING case and WORKING case. Can anyone help me to understand why a script that was working 2 weeks ago isn't working anymore? Thanks for your help Remo data(mtcars) mtcars xDist<-dist(mtcars,method="euclidean") xClust<-hclust(xDist,method="complete") plot(xClust,labels=FALSE) heatmap.2(t(mtcars), dendrogram=c("col"), Rowv=FALSE, Colv=as.dendrogram(xClust)) -- __________________________________________________________ Remo Chiozzotto PhD CNR (National Research Council) Institute of Agricultural Biology and Biotechnology (IBBA) Via E. Bassini, 15 - 20133 - Milano (Italy) Office +39 0223699427 Lab +39 0223699426 Fax +39 0223699411 e-mail: remo.chiozzotto at ibba.cnr.it skype: remo.chiozzotto __________________________________________________________ -- output of sessionInfo(): NOT WORKING R version 3.1.1 (2014-07-10) Platform: x86_64-pc-linux-gnu (64-bit) locale: [1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C LC_TIME=it_IT.UTF-8 LC_COLLATE=en_US.UTF-8 [5] LC_MONETARY=it_IT.UTF-8 LC_MESSAGES=en_US.UTF-8 LC_PAPER=it_IT.UTF-8 LC_NAME=C [9] LC_ADDRESS=C LC_TELEPHONE=C LC_MEASUREMENT=it_IT.UTF-8 LC_IDENTIFICATION=C attached base packages: [1] splines parallel stats graphics grDevices utils datasets methods base other attached packages: [1] NMF_0.20.5 Biobase_2.24.0 cluster_1.15.2 rngtools_1.2.4 pkgmaker_0.22 [6] registry_0.2 gplots_2.14.0 edgeR_3.6.4 limma_3.20.8 RColorBrewer_1.0-5 [11] ShortRead_1.22.0 GenomicAlignments_1.0.1 BSgenome_1.32.0 Rsamtools_1.16.1 GenomicRanges_1.16.3 [16] GenomeInfoDb_1.0.2 Biostrings_2.32.0 XVector_0.4.0 IRanges_1.22.9 BiocParallel_0.6.1 [21] BiocGenerics_0.10.0 loaded via a namespace (and not attached): [1] BatchJobs_1.2 BBmisc_1.7 bitops_1.0-6 brew_1.0-6 caTools_1.17 checkmate_1.1 [7] codetools_0.2-8 colorspace_1.2-4 DBI_0.2-7 digest_0.6.4 doParallel_1.0.8 fail_1.2 [13] foreach_1.4.2 gdata_2.13.3 ggplot2_1.0.0 grid_3.1.1 gridBase_0.4-7 gtable_0.1.2 [19] gtools_3.4.1 hwriter_1.3 iterators_1.0.7 KernSmooth_2.23-12 lattice_0.20-29 latticeExtra_0.6-26 [25] MASS_7.3-33 munsell_0.4.2 plyr_1.8.1 proto_0.3-10 Rcpp_0.11.2 reshape2_1.4 [31] RSQLite_0.11.4 scales_0.2.4 sendmailR_1.1-2 stats4_3.1.1 stringr_0.6.2 tools_3.1.1 [37] xtable_1.7-3 zlibbioc_1.10.0 WORKING R version 3.0.2 (2013-09-25) Platform: i386-w64-mingw32/i386 (32-bit) locale: [1] LC_COLLATE=Italian_Italy.1252 LC_CTYPE=Italian_Italy.1252 [3] LC_MONETARY=Italian_Italy.1252 LC_NUMERIC=C [5] LC_TIME=Italian_Italy.1252 attached base packages: [1] splines parallel stats graphics grDevices utils datasets [8] methods base other attached packages: [1] NMF_0.20.5 Biobase_2.22.0 cluster_1.14.4 [4] rngtools_1.2.4 pkgmaker_0.22 registry_0.2 [7] gplots_2.14.1 RColorBrewer_1.0-5 ShortRead_1.20.0 [10] Rsamtools_1.14.3 lattice_0.20-24 Biostrings_2.30.1 [13] GenomicRanges_1.14.4 XVector_0.2.0 IRanges_1.20.6 [16] BiocGenerics_0.8.0 BiocInstaller_1.12.1 edgeR_3.4.2 [19] limma_3.18.13 loaded via a namespace (and not attached): [1] bitops_1.0-6 caTools_1.17 codetools_0.2-8 [4] colorspace_1.2-4 digest_0.6.4 doParallel_1.0.8 [7] foreach_1.4.2 gdata_2.13.3 ggplot2_1.0.0 [10] grid_3.0.2 gridBase_0.4-7 gtable_0.1.2 [13] gtools_3.4.1 hwriter_1.3 iterators_1.0.7 [16] KernSmooth_2.23-10 latticeExtra_0.6-26 MASS_7.3-29 [19] munsell_0.4.2 plyr_1.8.1 proto_0.3-10 [22] Rcpp_0.11.2 reshape2_1.4 scales_0.2.4 [25] stats4_3.0.2 stringr_0.6.2 tools_3.0.2 [28] xtable_1.7-3 zlibbioc_1.8.0 -- Sent via the guest posting facility at bioconductor.org.
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Dan Du ▴ 210
@dan-du-5270
Last seen 11 months ago
Germany
Hi Remo, There is a bug in the gplots version you are using (2.14.0), which has been fixed in 2.14.1, so run an update should fix your problem, you example works fine for me. > Release 2.14.1 - 2014-06-30 > --------------------------- > > Bug Fixes: > > - Correct bug in Colv dentrogram object dimension checking introduced > in 2.14.0. (Reported by Yong Fuga Li.) HTH, Dan On Wed, 2014-07-16 at 06:35 -0700, Remo [guest] wrote: > Dear list, > I'm having issues in generating an heatmap which occurred only after the upgrade to ver. 3.1.1 > Below you find a very easy example which works in previous R version 3.0.2 but not in ver. 3.1.1 giving the following error. > > Error in heatmap.2(t(mtcars), dendrogram = c("col"), Rowv = FALSE, Colv = as.dendrogram(xClust)) : > Colv dendrogram doesn't match size of x > > I've also added sessionInfo() for the NOT WORKING case and WORKING case. > > Can anyone help me to understand why a script that was working 2 weeks ago isn't working anymore? > > Thanks for your help > > Remo > > > data(mtcars) > mtcars > > xDist<-dist(mtcars,method="euclidean") > xClust<-hclust(xDist,method="complete") > plot(xClust,labels=FALSE) > > heatmap.2(t(mtcars), > dendrogram=c("col"), > Rowv=FALSE, > Colv=as.dendrogram(xClust)) > >
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Thanks Dan, everything works smoothly again. I thought I had the last version but I was wrong. Ciao Remo On 16/07/2014 15:51, DaN wrote: > Hi Remo, > > There is a bug in the gplots version you are using (2.14.0), which has > been fixed in 2.14.1, so run an update should fix your problem, you > example works fine for me. > >> Release 2.14.1 - 2014-06-30 >> --------------------------- >> >> Bug Fixes: >> >> - Correct bug in Colv dentrogram object dimension checking introduced >> in 2.14.0. (Reported by Yong Fuga Li.) > HTH, > Dan > > On Wed, 2014-07-16 at 06:35 -0700, Remo [guest] wrote: >> Dear list, >> I'm having issues in generating an heatmap which occurred only after the upgrade to ver. 3.1.1 >> Below you find a very easy example which works in previous R version 3.0.2 but not in ver. 3.1.1 giving the following error. >> >> Error in heatmap.2(t(mtcars), dendrogram = c("col"), Rowv = FALSE, Colv = as.dendrogram(xClust)) : >> Colv dendrogram doesn't match size of x >> >> I've also added sessionInfo() for the NOT WORKING case and WORKING case. >> >> Can anyone help me to understand why a script that was working 2 weeks ago isn't working anymore? >> >> Thanks for your help >> >> Remo >> >> >> data(mtcars) >> mtcars >> >> xDist<-dist(mtcars,method="euclidean") >> xClust<-hclust(xDist,method="complete") >> plot(xClust,labels=FALSE) >> >> heatmap.2(t(mtcars), >> dendrogram=c("col"), >> Rowv=FALSE, >> Colv=as.dendrogram(xClust)) >> >> >
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