Hi, I thought I'd post this here, something to think about, especially if data from the latest chips lands on your desk. Had a seminar today from the affy rep - they are changing labelling kit/procedure from ENZO to IVT. The new one is better BUT you will not be able to directly compare between new and old. You will still be able to buy direct from ENZO to complete current projects. The biggest change appears to be they will switch to just using biotin-pseudouridine instead of biotin U and C. There are details below, but I can't find the really interesting figure he showed - which was a comparison of the PM and MM signals (of the spike-ins I guess) with ENZO (big overlap - as we know!) and with IVT (nice separation with PM significantly higher than MM). This will probably have a big impact on computing expression and detection. Something to keep us on our toes... http://www.affymetrix.com/support/technical/technotes/ivt_technote.pdf Also, better order the new computer as there could be alot of data - they are already down to 11?m from the previous 18?m, 5?m is on the way, and there is rumour of 1-2?m... Cheers, Matt