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Hello,
I have a problem with supplying GRanges object with seqlengths.
I have a files.
chrs.txt - contains information about chromosomes
chrs.txt
chr,start,end,len
lm_SuperContig_0_v2,1,4258568,4258568
lm_SuperContig_1_v2,1,3378610,3378610
lm_SuperContig_2_v2,1,2939989,2939989
lm_SuperContig_3_v2,1,2348246,2348246
lm_SuperContig_4_v2,1,1918205,1918205
lm_SuperContig_6_v2,1,1888674,1888674
lm_SuperContig_5_v2,1,1869450,1869450
lm_SuperContig_8_v2,1,1809296,1809296
lm_SuperContig_9_v2,1,1772623,1772623
lm_SuperContig_7_v2,1,1769547,1769547
lm_SuperContig_10_v2,1,1758670,1758670
lm_SuperContig_13_v2,1,1634580,1634580
lm_SuperContig_12_v2,1,1631710,1631710
lm_SuperContig_11_v2,1,1590160,1590160
lm_SuperContig_15_v2,1,1560629,1560629
lm_SuperContig_14_v2,1,1533332,1533332
lm_SuperContig_17_v2,1,1445693,1445693
lm_SuperContig_16_v2,1,1397653,1397653
lm_SuperContig_18_v2,1,1351976,1351976
lm_SuperContig_19_v2,1,1186800,1186800
lm_SuperContig_20_v2,1,1087932,1087932
lm_SuperContig_21_v2,1,1020521,1020521
lm_SuperContig_22_v2,1,731443,731443
lm_SuperContig_23_v2,1,521426,521426
lm_SuperContig_24_v2,1,475869,475869
lm_SuperContig_25_v2,1,318058,318058
lm_SuperContig_26_v2,1,261540,261540
lm_SuperContig_27_v2,1,250629,250629
lm_SuperContig_28_v2,1,236098,236098
lm_SuperContig_29_v2,1,200940,200940
lm_SuperContig_30_v2,1,154863,154863
lm_SuperContig_31_v2,1,143268,143268
lm_SuperContig_32_v2,1,87679,87679
lm_SuperContig_34_v2,1,58596,58596
I try to do the following:
# creating GRanges object for chromosomes
dataChr <- read.table("chrs.txt",header=T,sep=",")
chrs <- with(dataChr, GRanges(chr, IRanges(start, end)))
sl <- setNames(dataChr$len, as.character(dataChr$chr))
seqlengths(chrs) <- sl
And I get the following error:
Error in .normargSeqlengths(value, seqnames(x)) :
when the supplied 'seqlengths' vector is named, the names must match
the seqnames
Any chance you could help me with what is going on?
Best wishes,
Agnieszka
-- output of sessionInfo():
R version 3.1.0 (2014-04-10)
Platform: i386-w64-mingw32/i386 (32-bit)
locale:
[1] LC_COLLATE=English_United Kingdom.1252 LC_CTYPE=English_United
Kingdom.1252 LC_MONETARY=English_United Kingdom.1252
[4] LC_NUMERIC=C LC_TIME=English_United
Kingdom.1252
attached base packages:
[1] parallel stats graphics grDevices utils datasets
methods base
other attached packages:
[1] XVector_0.4.0 ggbio_1.12.3 ggplot2_0.9.3.1
GenomicRanges_1.16.2 GenomeInfoDb_1.0.2 IRanges_1.22.4
BiocGenerics_0.10.0
[8] BiocInstaller_1.14.2
loaded via a namespace (and not attached):
[1] AnnotationDbi_1.26.0 BatchJobs_1.2 BBmisc_1.6
Biobase_2.24.0 BiocParallel_0.6.0 biomaRt_2.20.0
[7] Biostrings_2.32.0 biovizBase_1.12.1 bitops_1.0-6
brew_1.0-6 BSgenome_1.32.0 cluster_1.15.2
[13] codetools_0.2-8 colorspace_1.2-4 DBI_0.2-7
dichromat_2.0-0 digest_0.6.4 fail_1.2
[19] foreach_1.4.2 Formula_1.1-1
GenomicAlignments_1.0.0 GenomicFeatures_1.16.0 grid_3.1.0
gridExtra_0.9.1
[25] gtable_0.1.2 Hmisc_3.14-4 iterators_1.0.7
labeling_0.2 lattice_0.20-29 latticeExtra_0.6-26
[31] MASS_7.3-31 munsell_0.4.2 plyr_1.8.1
proto_0.3-10 RColorBrewer_1.0-5 Rcpp_0.11.1
[37] RCurl_1.95-4.1 reshape2_1.4
Rsamtools_1.16.0 RSQLite_0.11.4 rtracklayer_1.24.0
scales_0.2.4
[43] sendmailR_1.1-2 splines_3.1.0 stats4_3.1.0
stringr_0.6.2 survival_2.37-7 tools_3.1.0
[49] VariantAnnotation_1.10.0 XML_3.98-1.1 zlibbioc_1.10.0
--
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