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Last seen 10.3 years ago
Hi,
I am trying to use the virtualArray package, using the example code in
the Vignette document, pasted below:
> library(GEOquery)
> library("hgu133plus2.db")
> library("hgug4112a.db")
> library(virtualArray)
>
> GSE23402 <-getGEO("GSE23402",GSEMatrix=T,AnnotGPL=FALSE)
> GSE26428 <-getGEO("GSE26428",GSEMatrix=T,AnnotGPL=FALSE)
> GSE23402 <- GSE23402[[1]][,1:24]
> GSE26428 <- GSE26428[[1]]
> exprs(GSE23402) <- log2(exprs(GSE23402))
> exprs(GSE26428) <- (exprs(GSE26428)/20*16)
>
> annotation(GSE23402) <- "hgu133plus2"
> annotation(GSE26428) <- "hgug4112a"
>
> my_virtualArrays <- NULL
> my_virtualArrays$iPSC_hESC_noBatchEffect
<-virtualArrayExpressionSets()
This code worked until about a week ago, but recently it gives me
warnings and errors. In particular, I get the following warning:
> Warning message:
> In download.file(myurl, destfile, mode = mode, quiet = >TRUE, method
= >getOption("download.file.method.GEOquery")): downloaded >length
64514067 != reported length 200
And also this error:
> Now preprocessing raw data of GSE2527: Annotating expression values
with SYMBOL...
> Error: 2 errors; first error:
> Error in array(x, c(length(x), 1L), if (!is.null(names(x)))
list(names(x), : 'data' must be of a > vector type, was 'NULL'
I am wondering if this issue is related to an error to the GEOquery
package or the virtualArray package. Is there something wrong with my
installation of R or any of my packages?
Regard,
Nafiseh
-- output of sessionInfo():
> sessionInfo()
R version 3.0.2 (2013-09-25)
Platform: x86_64-redhat-linux-gnu (64-bit)
attached base packages:
[1] parallel stats graphics grDevices utils datasets
methods base
other attached packages:
[1] hgu95av2.db_2.10.1 org.Hs.eg.db_2.10.1 RSQLite_0.11.4
DBI_0.2-7
[5] AnnotationDbi_1.24.0 BiocParallel_0.4.1 virtualArray_1.6.0
preprocessCore_1.24.0
[9] plyr_1.8.1 Biobase_2.22.0 BiocGenerics_0.8.0
loaded via a namespace (and not attached):
[1] affy_1.40.0 affyio_1.30.0 affyPLM_1.38.0
BatchJobs_1.2
[5] BBmisc_1.6 BiocInstaller_1.12.1 Biostrings_2.30.1
brew_1.0-6
[9] codetools_0.2-8 digest_0.6.4 fail_1.2
foreach_1.4.2
[13] gcrma_2.34.0 GEOquery_2.28.0 grid_3.0.2
IRanges_1.20.7
[17] iterators_1.0.7 lattice_0.20-29 outliers_0.14
quadprog_1.5-5
[21] Rcpp_0.11.1 RCurl_1.95-4.1 reshape2_1.4
sendmailR_1.1-2
[25] splines_3.0.2 stats4_3.0.2 stringr_0.6.2
tools_3.0.2
[29] tseries_0.10-32 XML_3.98-1.1 XVector_0.2.0
zlibbioc_1.8.0
[33] zoo_1.7-11
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