Thanks Lukas! That worked. I'll likely have more questions as the analysis continues...really appreciate your help. --Kelly From: Lukas Chavez [mailto:lukas.chavez.mailings@googlemail.com] Sent: Wednesday, December 18, 2013 12:42 PM To: Vining, Kelly Cc: bioconductor@r-project.org Subject: Re: [BioC] using custom BSgenome with MEDIPS Hi Kelly, let us assume that the package name of your successfully created custom BSgenome for e.g. 'oryza sativa' is 'BSgenome.OryzaSativa' and that it is installed in your R environment using R CMD INSTALL <package>. First load your custom BSgenome object into your R session using library(BSgenome.OryzaSativa) Note, BSgenome.OryzaSativa will not appear in the list returned by available.genomes(). However, in case the library is loaded you should be able to immediately use it as usual in MEDIPS like library(MEDIPS) BSgenome= "BSgenome.OryzaSativa" MSet = MEDIPS.createSet(file="oryza.bam", BSgenome=BSgenome) Lukas On Wed, Dec 18, 2013 at 6:57 AM, Vining, Kelly <kelly.vining@oregonstate.edu<mailto:kelly.vining@oregonstate.edu>> wrote: ________________________________ From: Vining, Kelly Sent: Tuesday, December 17, 2013 10:58 AM To: 'Lukas Chavez' Cc: bioconductor@r-project.org<mailto:bioconductor@r-project.org> Subject: RE: [BioC] Question about MEDIPS extend parameter Hello BioC group, have created a BSgenome object for my genome of interest, and it is in my current working directory. I now want to use it with MEDIPS. How do I make it appear under >available.genomes()? Is it necessary for my custom genome to appear in this list for me to be able to use it with MEDIPS? Or is there a different method for accessing this object? Thanks much, --Kelly V. [[alternative HTML version deleted]] _______________________________________________ Bioconductor mailing list Bioconductor@r-project.org<mailto:bioconductor@r-project.org> https://stat.ethz.ch/mailman/listinfo/bioconductor Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor [[alternative HTML version deleted]]