hi Mike, thanks a lot! That is what I have been looking for Dorota Michael Love wrote: > hi Dorota, > > I don't know if you are using DESeq or DESeq2, but in the latter I > have added an argument geoMeans to estimateSizeFactors which allows > you to provide the geometric means from a previous dataset to > normalize a new one. See the Examples section for ?estimateSizeFactors > for DESeq2 v1.2 > > Mike > > > On Thu, Nov 21, 2013 at 6:24 AM, Dorota Herman > <dorota.herman at="" psb.vib-ugent.be=""> <mailto:dorota.herman at="" psb.vib-ugent.be="">> wrote: > > Hello everyone, > > I have a question regarding normalization for a library size in > DESeq. I have a set of samples, which I had normalized with a > function estimateSizeFactors. Is there an option to normalized an > additional sample with respect to normalized previously set of > samples. I know that in the normalization in EdgeR treats a first > sample as a reference by default. How can I do it in DESeq? > > regards > Dorota > > > -- > ================================================================== > Dorota Herman, PhD > VIB Department of Plant Systems Biology, Ghent University > Technologiepark 927 > 9052 Gent, Belgium > Tel: +32 (0)9 3313692 <tel:%2b32%20%280%299%203313692> > Email:dorota.herman at psb.vib-ugent.be > <mailto:email%3adorota.herman at="" psb.vib-ugent.be=""> > Web: http://www.psb.ugent.be > > _______________________________________________ > Bioconductor mailing list > Bioconductor at r-project.org <mailto:bioconductor at="" r-project.org=""> > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor > >