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marchiem@libero.it
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@marchiemliberoit-547
Last seen 10.1 years ago
Hello everybody,
we are evaluating a new image analysis software called Blue Fuse that
uses a Bayesian approach to estimate the background. The outputs per
each channell of this software are values with the background already
subtracted.
How can I read in these output in BioC, specifically in limma?
I've used read.maimages and I've specified the columns just for
foreground signal and I put NULL for the background:
RGblue<-read.maimages(files3,columns=list(Rf="AMPCH2",Gf="AMPCH1",Rb=N
ULL,Gb=NULL))
but I received this error message:
Error in "[<-"(`*tmp*`, , i, value = structure(list(), .Names =
character(0), row.names = c("1", :
nothing to replace with
I do apologies but I'm not yet confident in R so I can't understand
how to solve the problem...
Maybe should I create another text file with two columns more for the
background and put zero in them?
Does anyone know how to read this kind of data?
Thanks in advance and sorry again if I made some trivial mistakes.
Regards.
Emanuele