Hi I have been trying to use DiffBind to analyze our Chip-seq data and have been running into some errors repeatedly. I first created a samplesheet.csv describing my samples and it looks like this: SampleID,Tissue,Factor,Condition,Replicate,bamReads,bamControl,Peaks,P eakCaller meio.1,meiocytes,H3K4me3,N,1,M_meiocytes_H3K4me3.bam,InM_input_meiocyt es.bam,meio.vs.in.rep1.def_peaks.bed,MACS seed.1,seedlings,H3K4me3,N,1,S_seedling_H3K4me3.bam,InS_input_seedling .bam,seed.vs.in.rep1.def_peaks.bed,MACS I only have two samples (and their respective inputs) with one rep each and the peaks were called using MACS v2. The peak caller generated .bed files which was used in DiffBind. I defined the working directory in R first. I then read the sample sheet in : > H3K4.B73=dba(sampleSheet='samplesheet2.csv',peakFormat='bed') >H3K4.B73 2 Samples, 38870 sites in matrix (45304 total): ID Tissue Factor Condition Replicate Peak.caller Intervals 1 meio.1 meiocytes H3K4me3 N 1 MACS 44124 2 seed.1 seedlings H3K4me3 N 1 MACS 41596 generated a plot, > plot(H3K4.B73) And then when I tried to perform dba.counts, it continuously fails on me. I went through the thread to find similar posts and could not find a solution. I tried the floowing command: > H3K4.B73=dba.count(H3K4.B73, minOverlap=3) and this, > H3K4.B73=dba.count(H3K4.B73, minOverlap=3, bLowMem=TRUE) > H3K4.B73=dba.count(H3K4.B73, minOverlap=3, bLowMem=FALSE) And they all failed. Please let me know if you have any insights on it. Thanks so much for your help in advance. Anitha Sundararajan Ph.D. Research Scientist National Center for Genome Resources Santa Fe, NM 87505