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Last seen 10.3 years ago
Hi, I am a graduate student at POSTECH and I ran into some troubles
while running edgeR program.
Currently I have six Chip-Seq samples, all of different condition.
There are no biological replicate and no control sample.
So when I try to run edgeR, it does not run for there is no biological
replicate used for statistical testing.
I wonder if there is a way to export TMM normalized count from edgeR
package without compliting the program.
I know the program should run up till calcNormFactor and give
normfactor but without biological replicate, it cannot calculate
dispersion and the whole program fails to run.
Is there a way to export just the TMM normalized data without doing
statistical testing?
If so, what are the codes that allow such methods?
I tried cpm function but cpm may use raw or normalized data not gives
a normalized data from raw data.
Thanks in advance,
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