Hi,Stevan, This warning message is due to the fact that R doesn't handle unsigned integer of size larger(or equal) than 4 Bytes. If your raw files store data as non-integer type (float or double),you can safely ignore this warning. Let me know if you have more questions regarding to flowCore. Mike Jiang,PhD System Analyst PS Statistics, Vaccine and Infectious Disease Division Fred Hutchinson Cancer Research Center (206)667-1905 On 10/19/2012 12:01 PM, Stevan Lauriault wrote: > > Hi, > > I've been given the assignment of reviewing the flowCore package for > the core flow facility here in Toronto and I'm encountering a few issues. > > First, when calling the read.FCS function to read a flow file: > > STL1 <- read.FCS("/users/steve/desktop/AMLX/Specimen_002_CD3.fcs", > transformation = FALSE) > > the following message is returned: > > Warning message: > In readBin(con = con, what = dattype, n = count, size = size, signed = > signed, : > 'signed = FALSE' is only valid for integers of sizes 1 and 2 > > I've tried multiple files and the same message is returned. Any ideas? > > Regards, >