Hi Juan, > Dear Heidi, > > My name is Juan Fernandez-Tajes and I?m using HTqPCR for analyzing some > Biomark 96*96 data. When I tried the following command I got an error: > >>raw1 <- readCtData(files="Prueba_Blanco001.txt", format="BioMark", >> n.features=96, n.data=96) > Error en `$<-.data.frame`(`*tmp*`, "Call", value = character(0)) : > replacement has 0 rows, data has 9216 > > However when I import the data with the following command it works: > >>temp <- readCtData(files="Prueba_Blanco001.txt", n.features=96*96, >> column.info=list(flag=9, feature=5, type=6, Ct=7, position=1), skip=12, >> sep="\t") >>raw <- changeCtLayout(temp, sample.order=rep(1:96, each=96)) > > Any ideas? > Not at the top of my head. However, you actually change two things here, namely both the specification of n.features/n.data and using 'format' vs 'column.info'. To help me figure out which of these two things causes the error, can you try running the remaining 2 combinations? I.e.: temp <- readCtData(files="Prueba_Blanco001.txt", n.features=96*96, format="BioMark") raw1 <- readCtData(files="Prueba_Blanco001.txt", n.features=96, n.data=96, column.info=list(flag=9, feature=5, type=6, Ct=7, position=1), skip=12, sep="\t") Thanks \Heidi > Many thanks in advance > > Juan > > --------------------------------------------------------------- > Juan Fernandez Tajes, ph. D > Grupo XENOMAR > Departamento de Biolog?a Celular y Molecular > Facultad de Ciencias-Universidade da Coru?a > Tlf. +34 981 167000 ext 2030 > e-mail: jfernandezt at udc.es > ---------------------------------------------------------------- > > >