hello eveybody, I'd like to know your opinion about how to analyze time-serie experiments with BioC. We use a common reference (gDNA) at each time point. According to you wich is the simplest method to analyse this experiment? the anova function in genefilter package would be enough or should I use limma? I do apologises if the questions is trivial for you. Emanuele

Hello everybody, I have encountered a problem with the pamr.from.excel function. When I try to perform this function on my file it seems that I have 781 genes whereas my files has 1000 genes. When I compare the geneid obtained and those who are present in my file I make the remark that some genes have been removed by groups of around 20. I mean that I have the 100 first genes and then the 20 following genes are removed and so on..... I really don't understand what happened ! For details I had : data<-pamr.from.excel("file.txt",90,sample.labels=TRUE,batch.labels= FALSE) Read 70470 items Read in 781 genes Read in 88 samples Read in 88 sample labels Make sure these figures are correct!! Could you help me ? Thank you, Regards Willy