Entering edit mode
Guest User
★
13k
@guest-user-4897
Last seen 10.2 years ago
sir,
i am new on R language and dealing with light-cycler qpcr data using
HtqPCR package.everything goes all right till fold change.
when i use t-test then following error occured.
> qDE.ttest <- ttestCtData(sr.norm[, 1:2], groups =
files$Treatment[1:2], calibrator = "Control")
Error in t.test.default(x[, g1], x[, g2], alternative = alternative,
paired = paired, :
data are essentially constant
when i select 4 samples then
qDE.ttest <- ttestCtData(sr.norm[, 1:4], groups =
files$Treatment[1:4], calibrator = "Control")
Error in ttestCtData(sr.norm[, 1:4], groups = files$Treatment[1:4],
calibrator = "Control") :
Two factor levels required for 'groups'
my parent file look like this
File Treatment
control.txt Control
30min.txt 30min
2hr.txt 2hr
4hr.txt 4hr
8hr.txt 8hr
12hr.txt 12hr
16hr.txt 16hr
24hr.txt 24hr
48hr.txt 48hr
every sample have 26 features(13 replicate)
so how i perform t- test on these data.
please sir help me out.
thanking you
-- output of sessionInfo():
R version 2.15.1 (2012-06-22)
Platform: i386-pc-mingw32/i386 (32-bit)
locale:
[1] LC_COLLATE=English_United States.1252 LC_CTYPE=English_United
States.1252 LC_MONETARY=English_United States.1252
[4] LC_NUMERIC=C LC_TIME=English_United
States.1252
attached base packages:
[1] stats graphics grDevices utils datasets methods base
other attached packages:
[1] HTqPCR_1.10.0 limma_3.12.1 RColorBrewer_1.0-5
Biobase_2.16.0 BiocGenerics_0.2.0
loaded via a namespace (and not attached):
[1] affy_1.34.0 affyio_1.24.0 BiocInstaller_1.4.7
gdata_2.11.0 gplots_2.11.0
[6] gtools_2.7.0 preprocessCore_1.18.0 stats4_2.15.1
tools_2.15.1 zlibbioc_1.2.0
--
Sent via the guest posting facility at bioconductor.org.