Hi all, I use qPCR array of 384 wells and I normalize using d.norm. But I used different RNA input in every sample, so I thinks the differentially expressed genes are not right. I think I need to normalize in another way before d.norm, but I don't know how to do it. How may I found the more accurate method? Thank you in advance. Atte, Carmen Palacios. [[alternative HTML version deleted]]

Hi Carmen, A few questions to get a better idea of the experiment. Firstly, what do you mean by d norm? delta Cq method? I.e. subtraction of reference genes? How many samples do you have? How many conditions? and how many genes in each samples? You say you have different RNA input in every sample, what do you mean by this? Do you have biological replication? Cheers, Jim On 25 July 2012 23:09, Carmen Palacios <cyapalacios@gmail.com> wrote: > Hi all, > > I use qPCR array of 384 wells and I normalize using d.norm. > But I used different RNA input in every sample, so I thinks the > differentially expressed genes are not right. > I think I need to normalize in another way before d.norm, but I don't know > how to do it. > How may I found the more accurate method? > > Thank you in advance. > > Atte, Carmen Palacios. > > [[alternative HTML version deleted]] > > _______________________________________________ > Bioconductor mailing list > Bioconductor@r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor > [[alternative HTML version deleted]]