Entering edit mode
Dear Jorge,
Please see the vignette "APTvsXPS.pdf" for a comparison between APT
and
xps, and see Figure 25 for the reason why xps did not implement PLIER.
I do not think that you can import the APT output into xps, at least
not
from within R (although it might be possible from C++, but I would
have
to check).
Best regards
Christian
On 6/2/12 5:20 PM, Jorge Mir? wrote:
> Hi Christian,
>
> Oh, very nice. Is there any way of running PLIER in XPS too? Or is
there
> any possibility of producing the expressions with Affymetrix APT and
> then use the output files in XPS to do a quality analysis within
XPS?
>
> Kindly
> Jorge
>
> On Sat, Jun 2, 2012 at 5:14 PM, cstrato <cstrato at="" aon.at=""> <mailto:cstrato at="" aon.at="">> wrote:
>
> Dear Jorge,
>
> You can preprocess both, RaExon and RaGene 1.0 ST arrays, at the
> probeset and the gene level, simply set the option to either
> option="probeset" or to option="transcript", e.g.:
>
> data.rma <- rma(data.genome ,"RaGeneRMAMetacore", filedir=datdir
> background="antigenomic", normalize=TRUE, option="transcript",
> exonlevel="metacore+affx")
>
> See the help file ?rma.
>
> For further examples see the scripts "script4exon.R" and
> "script4xps.R" in the xps/examples directory. See also script
> "script4schemes.R" how to create the scheme for the RaGene
array.
>
> Best regards
> Christian
> _._._._._._._._._._._._._._._.___._._
> C.h.r.i.s.t.i.a.n S.t.r.a.t.o.w.a
> V.i.e.n.n.a A.u.s.t.r.i.a
> e.m.a.i.l: cstrato at aon.at <http: aon.at="">
> _._._._._._._._._._._._._._._.___._._
>
>
>
>
>
> On 6/2/12 3:02 AM, Jorge Mir? [guest] wrote:
>
>
> I was wondering if there is some way of getting the XPS
package
> working at gene level as I need to get the gene expression
from
> some Rat Gene chips (RaGene 1.0 ST r4)that I will analyze.
>
> I tried to use the Affy package before but as far as I
> understand they need .CDF file to get working and I only
have
> CLF and PBG files for my chips.
>
> Kind regards
> Jorge
>
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