Hai, I had few questions regarding the analysis of Agilent microarray data using the LIMMA pacakge. Is it possible to analyse the single color data using the R+Bioconductor using the LIMMA package? We dont have the replicates instead of that we have pooled the samples before sending for the analysis. So is it possible to use the LIMMA package without having any replicates. -- [[alternative HTML version deleted]]

On 4/27/2012 1:48 AM, Muralidharan V wrote: > Hai, > > I had few questions regarding the analysis of Agilent microarray data > using the LIMMA pacakge. > > Is it possible to analyse the single color data using the R+Bioconductor > using the LIMMA package? We dont have the replicates instead of that we > have pooled the samples before sending for the analysis. > So is it possible to use the LIMMA package without having any replicates. You can analyze single color Agilent microarray data with limma. However, if you don't have replicates you cannot do anything more than compute fold change (which being trivial, is not something that limma does). Best, Jim > > > -- > > [[alternative HTML version deleted]] > > _______________________________________________ > Bioconductor mailing list > Bioconductor at r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor -- James W. MacDonald, M.S. Biostatistician University of Washington Environmental and Occupational Health Sciences 4225 Roosevelt Way NE, # 100 Seattle WA 98105-6099