I'm not sure about the library size you mentioned. > Is it the total reads of a sample or the sum of the reads mapped to each gene. > I mean, if the total reads of a sample is 1,000,100, > but when I mapped the reads to the gene, I got a list of gene with reads mapped to it, and the sum of this is 900,000. > Do I use 1,000,000 or 900,000?? [[alternative HTML version deleted]]