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Francois Collin
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@francois-collin-657
Last seen 10.2 years ago
Anybody know of published studies assessing the contribution to
expression variability of the various parts of the GeneChip expression
data production process - RNA isolation, labeled target preparation,
Hybe+wash+scan+analysis?
There are two related reports on the Affy web site comparing GeneChip
output - focusing on presents calls, and comparisons of absolute
expression of genes of unknown true expression level:
1. An Analysis of Blood Processing Methods to Prepare Samples for
GeneChipŽ Expression Profiling
2. Globin Reduction Protocol: A Method for Processing Whole Blood RNA
Samples for Improved Array Results
Are there other published studies on this topic (doesn't have to be
blood - any source of RNA that can be treated with alternative RNA
isolation protocols would present the same problem of deciding which
protocol is best for a given application)?
Thanks in advance.
-Francois
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