Hi Gordon and Bioconductor patrons, I have 6 experimental groups with 3 biological replicates each and I used the function estimateGLMTagwiseDisp to look at dispersion estimates for my data. When I plotted tags vs abundance, I see a strange shape (data artifact ). Would you please help me understand what property of the data can cause this? > plot(y$abundance,y$tagwise.dispersion) https://docs.google.com/file/d/0B_mk9qnFziV- ZFBhak5fUGZSZ09VV0dJQ21Id1hYQQ/edit Thanks, Al [[alternative HTML version deleted]]