Hi, I downloaded limmaGUI and loaded to RGUI. These are the messages I got: Loading required package: tcltk Loading required package: limma ActiveTcl was found in the Windows Registry (for LOCAL_MACHINE), installed in D:\PROGRAM FILES\Tcl This directory will be added to the Tcl search path to enable limmaGUI to find the Tktable and BWidget extensions. limmaGUI can be launched by typing limmaGUI() or by using the pull- down menu. It seems to be OK at the beginning but when I tried to initiate the graphical interface I got a set of error such as ".. Error in paste(parent$ID,evalq(num.subwin<-numsubwin+1,parent$env),: Object"ttMain" not found." Then limma suggest me to close the GUI because it probably won't work well. Any ideas what to do? Thank, Ron [[alternative HTML version deleted]]