Dear All, Dear Christian,
I'm using XPS to process/analyse human Exon arrays.
I would like to use fRMA (frozen Robust MultiArray Analysis) to
normalize/summarize the data.
I'd like to know if is there a way to summarize/normalize data using
fRMA in
XPS.
More generally, is there a way to process the data by other methods
(non
supported by XPS) and then use it in the XPS framework?
Cheers,
Thomas
[[alternative HTML version deleted]]
Dear Thomas,
I was looking at the source code of fRMA, which currently supports
only
"hgu133a", "hgu133plus2", "mouse4302" arrays, and realized that it is
not possible for me to support fRMA, since it depends on pre-
calculated
public-domain data which you need, e.g. hgu133afrmavecs. For this
reason
I cannot support fRMA. However, keep in mind that fRMA still uses RMA
as
algorithm.
Best regards
Christian
On 9/24/11 1:32 AM, thomas sierocinski wrote:
> Dear All, Dear Christian,
>
> I'm using XPS to process/analyse human Exon arrays.
> I would like to use fRMA (frozen Robust MultiArray Analysis) to
> normalize/summarize the data.
> I'd like to know if is there a way to summarize/normalize data using
> fRMA in XPS.
> More generally, is there a way to process the data by other methods
(non
> supported by XPS) and then use it in the XPS framework?
>
> Cheers,
>
> Thomas
>
>
>
Dear Chrisitan,
Thanks for your answer.
Is there any way to assign a data matrix (created outside XPS) to a
ROOT
tree?
Cheers,
Thomas
2011/9/24 cstrato <cstrato@aon.at>
> Dear Thomas,
>
> I was looking at the source code of fRMA, which currently supports
only
> "hgu133a", "hgu133plus2", "mouse4302" arrays, and realized that it
is not
> possible for me to support fRMA, since it depends on pre-calculated
> public-domain data which you need, e.g. hgu133afrmavecs. For this
reason I
> cannot support fRMA. However, keep in mind that fRMA still uses RMA
as
> algorithm.
>
> Best regards
> Christian
>
>
>
> On 9/24/11 1:32 AM, thomas sierocinski wrote:
>
>> Dear All, Dear Christian,
>>
>> I'm using XPS to process/analyse human Exon arrays.
>> I would like to use fRMA (frozen Robust MultiArray Analysis) to
>> normalize/summarize the data.
>> I'd like to know if is there a way to summarize/normalize data
using
>> fRMA in XPS.
>> More generally, is there a way to process the data by other methods
(non
>> supported by XPS) and then use it in the XPS framework?
>>
>> Cheers,
>>
>> Thomas
>>
>>
>>
>>
[[alternative HTML version deleted]]
Dear Thomas,
Yes, it is possible, see the examples in the help files ?'intensity<-'
and ?'expr<-'.
ad 1, intensity<-
You can create new raw data, e.g. a user of xps has once applied "vsn"
to the raw data and then imported the result into DataTreeSet using
'intensity<-'. However, be careful since CEL-files will be created,
see
the help file.
ad 2, expr<-
In principle it is possible to import any result of preprocessing into
an ExprTreeSet. For example you could use fRMA and then import the
result using 'expr<-'.
I hope that this is the information you are looking for, however it
would be helpful if you could explain in more detail what you would
like
to do.
Best regards
Christian
On 9/27/11 10:04 PM, thomas sierocinski wrote:
> Dear Chrisitan,
>
> Thanks for your answer.
>
> Is there any way to assign a data matrix (created outside XPS) to a
ROOT
> tree?
>
> Cheers,
>
> Thomas
>
> 2011/9/24 cstrato <cstrato at="" aon.at="" <mailto:cstrato="" at="" aon.at="">>
>
> Dear Thomas,
>
> I was looking at the source code of fRMA, which currently
supports
> only "hgu133a", "hgu133plus2", "mouse4302" arrays, and realized
that
> it is not possible for me to support fRMA, since it depends on
> pre-calculated public-domain data which you need, e.g.
> hgu133afrmavecs. For this reason I cannot support fRMA. However,
> keep in mind that fRMA still uses RMA as algorithm.
>
> Best regards
> Christian
>
>
>
> On 9/24/11 1:32 AM, thomas sierocinski wrote:
>
> Dear All, Dear Christian,
>
> I'm using XPS to process/analyse human Exon arrays.
> I would like to use fRMA (frozen Robust MultiArray Analysis)
to
> normalize/summarize the data.
> I'd like to know if is there a way to summarize/normalize
data using
> fRMA in XPS.
> More generally, is there a way to process the data by other
> methods (non
> supported by XPS) and then use it in the XPS framework?
>
> Cheers,
>
> Thomas
>
>
>
>
This is very helpful. Thanks.
Thomas
2011/9/27 cstrato <cstrato@aon.at>
> Dear Thomas,
>
> Yes, it is possible, see the examples in the help files
?'intensity<-' and
> ?'expr<-'.
>
> ad 1, intensity<-
> You can create new raw data, e.g. a user of xps has once applied
"vsn" to
> the raw data and then imported the result into DataTreeSet using
> 'intensity<-'. However, be careful since CEL-files will be created,
see the
> help file.
>
> ad 2, expr<-
> In principle it is possible to import any result of preprocessing
into an
> ExprTreeSet. For example you could use fRMA and then import the
result using
> 'expr<-'.
>
> I hope that this is the information you are looking for, however it
would
> be helpful if you could explain in more detail what you would like
to do.
>
> Best regards
> Christian
>
>
>
> On 9/27/11 10:04 PM, thomas sierocinski wrote:
>
>> Dear Chrisitan,
>>
>> Thanks for your answer.
>>
>> Is there any way to assign a data matrix (created outside XPS) to a
ROOT
>> tree?
>>
>> Cheers,
>>
>> Thomas
>>
>> 2011/9/24 cstrato <cstrato@aon.at <mailto:cstrato@aon.at="">>
>>
>>
>> Dear Thomas,
>>
>> I was looking at the source code of fRMA, which currently
supports
>> only "hgu133a", "hgu133plus2", "mouse4302" arrays, and realized
that
>> it is not possible for me to support fRMA, since it depends on
>> pre-calculated public-domain data which you need, e.g.
>> hgu133afrmavecs. For this reason I cannot support fRMA. However,
>> keep in mind that fRMA still uses RMA as algorithm.
>>
>> Best regards
>> Christian
>>
>>
>>
>> On 9/24/11 1:32 AM, thomas sierocinski wrote:
>>
>> Dear All, Dear Christian,
>>
>> I'm using XPS to process/analyse human Exon arrays.
>> I would like to use fRMA (frozen Robust MultiArray Analysis)
to
>> normalize/summarize the data.
>> I'd like to know if is there a way to summarize/normalize
data
>> using
>> fRMA in XPS.
>> More generally, is there a way to process the data by other
>> methods (non
>> supported by XPS) and then use it in the XPS framework?
>>
>> Cheers,
>>
>> Thomas
>>
>>
>>
>>
>>
--
-----------------------------------------------------------
Thomas Sierocinski, PhD
Genomic Analyst
GenomeDx Biosciences Inc. | 201-1595 W. 3rd Ave
Vancouver, BC V6J 1J8 | Canada
*Phone: 604-569-0072 | Fax: 866-505-5161*
email: *thomas@genomedx.com*
http://www.genomedx.com
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